Cell Communication in a Coculture System Consisting of Outgrowth Endothelial Cells and Primary Osteoblasts
المؤلفون المشاركون
Kirkpatrick, Charles James
Bischoff, Iris
Herzog, David Paul Eric
Dohle, Eva
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-22
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Bone tissue is a highly vascularized and dynamic system with a complex construction.
In order to develop a construct for implant purposes in bone tissue engineering, a proper understanding of the complex dependencies between different cells and cell types would provide further insight into the highly regulated processes during bone repair, namely, angiogenesis and osteogenesis, and might result in sufficiently equipped constructs to be beneficial to patients and thereby accomplish their task.
This study is based on an in vitro coculture model consisting of outgrowth endothelial cells and primary osteoblasts and is currently being used in different studies of bone repair processes with special regard to angiogenesis and osteogenesis.
Coculture systems of OECs and pOBs positively influence the angiogenic potential of endothelial cells by inducing the formation of angiogenic structures in long-term cultures.
Although many studies have focused on cell communication, there are still numerous aspects which remain poorly understood.
Therefore, the aim of this study is to investigate certain growth factors and cell communication molecules that are important during bone repair processes.
Selected growth factors like VEGF, angiopoietins, BMPs, and IGFs were investigated during angiogenesis and osteogenesis and their expression in the cultures was observed and compared after one and four weeks of cultivation.
In addition, to gain a better understanding on the origin of different growth factors, both direct and indirect coculture strategies were employed.
Another important focus of this study was to investigate the role of “gap junctions,” small protein pores which connect adjacent cells.
With these bridges cells are able to exchange signal molecules, growth factors, and other important mediators.
It could be shown that connexins, the gap junction proteins, were located around cell nuclei, where they await their transport to the cell membrane.
In addition, areas in which two cells formed gap junctions were found.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Herzog, David Paul Eric& Dohle, Eva& Bischoff, Iris& Kirkpatrick, Charles James. 2014. Cell Communication in a Coculture System Consisting of Outgrowth Endothelial Cells and Primary Osteoblasts. BioMed Research International،Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-463232
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Herzog, David Paul Eric…[et al.]. Cell Communication in a Coculture System Consisting of Outgrowth Endothelial Cells and Primary Osteoblasts. BioMed Research International No. 2014 (2014), pp.1-15.
https://search.emarefa.net/detail/BIM-463232
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Herzog, David Paul Eric& Dohle, Eva& Bischoff, Iris& Kirkpatrick, Charles James. Cell Communication in a Coculture System Consisting of Outgrowth Endothelial Cells and Primary Osteoblasts. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-15.
https://search.emarefa.net/detail/BIM-463232
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463232
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر