Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes
المؤلفون المشاركون
Huang, Chun-Yin
Tang, Chih-Hsin
Hsu, Horng-Chaung
Cheng, Shi-Yann
Chen, Hsin-Shui
Lin, Hsiu-Jung
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-09
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Thrombin is a key mediator of fibrin deposition, angiogenesis, and proinflammatory processes.
Abnormalities in these processes are primary features of rheumatoid arthritis and osteoarthritis.
Matrix metalloproteinase-13 (MMP-13) may contribute to the breakdown of articular cartilage during arthritis.
However, the role of thrombin in MMP-13 production in chondrocytes is unknown.
In this study, we investigated the intracellular signaling pathways involved in thrombin-induced MMP-13 expression in human chondrocytes.
We found that stimulation with thrombin led to increased secretion of MMP-13 in cultured human chondrocytes.
Further, this thrombin-induced MMP-13 production was reduced after transfection with siRNAs against protease activated receptors 1 and 3 (PAR1 and PAR3), but not with PAR4 siRNA.
Treatment with specific inhibitors for PKCδ, c-Src, EGFR, PI3K, Akt, or AP-1 or with the corresponding siRNAs against these signaling proteins also abolished the thrombin-mediated increase in MMP-13 production in chondrocytes.
Our results provide evidence that thrombin acts through the PAR1/PAR3 receptors and activates PKCδ and c-Src, resulting in EGFR transactivation and activation of PI3K, Akt, and finally AP-1 on the MMP-13 promoter, thereby contributing to cartilage destruction during arthritis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Chun-Yin& Lin, Hsiu-Jung& Chen, Hsin-Shui& Cheng, Shi-Yann& Hsu, Horng-Chaung& Tang, Chih-Hsin. 2013. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Chun-Yin…[et al.]. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Chun-Yin& Lin, Hsiu-Jung& Chen, Hsin-Shui& Cheng, Shi-Yann& Hsu, Horng-Chaung& Tang, Chih-Hsin. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463702
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر