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Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes
المؤلفون المشاركون
Huang, Chun-Yin
Tang, Chih-Hsin
Hsu, Horng-Chaung
Cheng, Shi-Yann
Chen, Hsin-Shui
Lin, Hsiu-Jung
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-09
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Thrombin is a key mediator of fibrin deposition, angiogenesis, and proinflammatory processes.
Abnormalities in these processes are primary features of rheumatoid arthritis and osteoarthritis.
Matrix metalloproteinase-13 (MMP-13) may contribute to the breakdown of articular cartilage during arthritis.
However, the role of thrombin in MMP-13 production in chondrocytes is unknown.
In this study, we investigated the intracellular signaling pathways involved in thrombin-induced MMP-13 expression in human chondrocytes.
We found that stimulation with thrombin led to increased secretion of MMP-13 in cultured human chondrocytes.
Further, this thrombin-induced MMP-13 production was reduced after transfection with siRNAs against protease activated receptors 1 and 3 (PAR1 and PAR3), but not with PAR4 siRNA.
Treatment with specific inhibitors for PKCδ, c-Src, EGFR, PI3K, Akt, or AP-1 or with the corresponding siRNAs against these signaling proteins also abolished the thrombin-mediated increase in MMP-13 production in chondrocytes.
Our results provide evidence that thrombin acts through the PAR1/PAR3 receptors and activates PKCδ and c-Src, resulting in EGFR transactivation and activation of PI3K, Akt, and finally AP-1 on the MMP-13 promoter, thereby contributing to cartilage destruction during arthritis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Huang, Chun-Yin& Lin, Hsiu-Jung& Chen, Hsin-Shui& Cheng, Shi-Yann& Hsu, Horng-Chaung& Tang, Chih-Hsin. 2013. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Huang, Chun-Yin…[et al.]. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Huang, Chun-Yin& Lin, Hsiu-Jung& Chen, Hsin-Shui& Cheng, Shi-Yann& Hsu, Horng-Chaung& Tang, Chih-Hsin. Thrombin Promotes Matrix Metalloproteinase-13 Expression through the PKCδc-SrcEGFRPI3KAktAP-1 Signaling Pathway in Human Chondrocytes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-463702
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-463702
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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