Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad
المؤلفون المشاركون
Zhu, Hua-Bin
Lin, Xiu-Kun
Wu, Ning
Yu, Ai-Bing
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-02-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Delivering siRNA or shRNA into the developing embryos is still a main challenge to use of RNAi in mammalian systems.
Here we analyze several factors influencing RNAi-mediated silencing of Sry gene, which is a tightly controlled spatiotemporal expressed gene and only shortly expressed in developing mouse embryo gonad.
A Sry gene-specific shRNAs expression vector (pSilencer4.1/Sry565) was constructed.
The shRNA constructs were mixed with polyethylenimines (PEIs) to form a complex and then injected into pregnant mice though tail vein.
Our results showed that Sry gene was downregulated significantly in developing embryos.
Further study revealed that knocking-down of Sry expression resulted in feminization of gonad development in mouse embryos and the expression level of Sox9 and Wt1 gene was also significantly changed by downregulation of Sry.
The transfection efficiency is associated with the amount of plasmid DNA injection, injection time, injection speed, and volume.
Our studies suggest that transplacental RNAi could be implemented by tail vein injection of plasmid vector into pregnant mice.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Ning& Yu, Ai-Bing& Zhu, Hua-Bin& Lin, Xiu-Kun. 2012. Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-464432
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Ning…[et al.]. Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-464432
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Ning& Yu, Ai-Bing& Zhu, Hua-Bin& Lin, Xiu-Kun. Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-464432
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464432
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر