Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells
المؤلفون المشاركون
Capela, João Paulo
Carvalho, Félix
Martins, João Barbosa
Bastos, Maria de Lourdes
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-03-20
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
العلوم الطبية والصيدلة والعلوم الصحية
الطب البشري
الملخص EN
Paraquat (PQ), a cationic nonselective bipyridyl herbicide, has been used as neurotoxicant to modulate Parkinson’s disease in laboratory settings.
Other compounds like rotenone (ROT), a pesticide, and 1-methyl-4-phenylpyridinium ion (MPP+) have been widely used as neurotoxicants.
We compared the toxicity of these three neurotoxicants using differentiated dopaminergic SH-SY5Y human cells, aiming to elucidate their differential effects.
PQ-induced neurotoxicity was shown to be concentration and time dependent, being mitochondrial dysfunction followed by neuronal death.
On the other hand, cells exposure to MPP+ induced mitochondrial dysfunction, but not cellular lyses.
Meanwhile, ROT promoted both mitochondrial dysfunction and neuronal death, revealing a biphasic pattern.
To further elucidate PQ neurotoxic mechanism, several protective agents were used.
SH-SY5Y cells pretreatment with tiron (TIR) and 2-hydroxybenzoic acid sodium salt (NaSAL), both antioxidants, and Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor, partially protected against PQ-induced cell injury.
Additionally, 1-(2-[bis(4-fluorophenyl)methoxy]ethyl)-4-(3-phenyl-propyl)piperazine (GBR 12909), a dopamine transporter inhibitor, and cycloheximide (CHX), a protein synthesis inhibitor, also partially protected against PQ-induced cell injury.
In conclusion, we demonstrated that PQ, MPP+, and ROT exerted differential toxic effects on dopaminergic cells.
PQ neurotoxicity occurred through exacerbated oxidative stress, with involvement of uptake through the dopamine transporter and protein synthesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martins, João Barbosa& Bastos, Maria de Lourdes& Carvalho, Félix& Capela, João Paulo. 2013. Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells. Journal of Toxicology،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-464636
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martins, João Barbosa…[et al.]. Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells. Journal of Toxicology No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-464636
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martins, João Barbosa& Bastos, Maria de Lourdes& Carvalho, Félix& Capela, João Paulo. Differential Effects of Methyl-4-Phenylpyridinium Ion, Rotenone, and Paraquat on Differentiated SH-SY5Y Cells. Journal of Toxicology. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-464636
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-464636
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر