Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation
المؤلفون المشاركون
Garlapati, Vijay Kumar
Chauhan, Rajinder Singh
Chauhan, Mamta
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Microbial enzymes from extremophilic regions such as hot spring serve as an important source of various stable and valuable industrial enzymes.
The present paper encompasses the modeling and optimization approach for production of halophilic, solvent, tolerant, and alkaline lipase from Staphylococcus arlettae through response surface methodology integrated nature inspired genetic algorithm.
Response surface model based on central composite design has been developed by considering the individual and interaction effects of fermentation conditions on lipase production through submerged fermentation.
The validated input space of response surface model (with R2 value of 96.6%) has been utilized for optimization through genetic algorithm.
An optimum lipase yield of 6.5 U/mL has been obtained using binary coded genetic algorithm predicted conditions of 9.39% inoculum with the oil concentration of 10.285% in 2.99 hrs using pH of 7.32 at 38.8°C.
This outcome could contribute to introducing this extremophilic lipase (halophilic, solvent, and tolerant) to industrial biotechnology sector and will be a probable choice for different food, detergent, chemical, and pharmaceutical industries.
The present work also demonstrated the feasibility of statistical design tools integration with computational tools for optimization of fermentation conditions for maximum lipase production.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chauhan, Mamta& Chauhan, Rajinder Singh& Garlapati, Vijay Kumar. 2013. Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation. Enzyme Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-465261
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chauhan, Mamta…[et al.]. Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation. Enzyme Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-465261
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chauhan, Mamta& Chauhan, Rajinder Singh& Garlapati, Vijay Kumar. Modelling and Optimization Studies on a Novel Lipase Production by Staphylococcus arlettae through Submerged Fermentation. Enzyme Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-465261
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465261
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر