RhC Phenotyping, AdsorptionElution Test, and SSP-PCR : The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors
المؤلفون المشاركون
Srijinda, Songsak
Suwanasophon, Chamaiporn
Pongsavee, Malinee
Visawapoka, Unchalee
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-14
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The Rhesus (Rh) blood group is the most polymorphic human blood group and it is clinically significant in transfusion medicine.
Especially, D antigen is the most important and highly immunogenic antigen.
Due to anti-D, it is the cause of the hemolytic disease of the newborn and transfusion reaction.
About 0.1%–0.5% of Asian people are RhD-negative, whereas in the Thai population, the RhD-negative blood type only occurs in 0.3%.
Approximately 10%–30% of RhD-negative in Eastern Asian people actually were D-elute (DEL) phenotype, the very weak D antigen that cannot be detected by indirect antiglobulin test (IAT).
There are many reports about anti-D immunization in RhD-negative recipients through the transfusion of red blood cells from individuals with DEL phenotype.
D-elute phenotype screening in Thai RhD-negative blood donors was studied to distinguish true RhD-negative from DEL phenotype.
A total of 254 Thai serologically RhD-negative blood donors were tested for RhCE phenotypes and anti-D adsorption/elution test.
In addition, RhC(+) samples were tested for RHD 1227A allele by SSP-PCR technique.
The RhD-negative phenotype samples consisted of 131 ccee, 4 ccEe, 1 ccEE, 101 Ccee, 16 CCee, and 1 CcEe.
The 42 Ccee and 8 CCee phenotype samples were typed as DEL phenotype and 96% of DEL samples were positive for RHD 1227A allele.
The incidence of RhC(+) was 46.4%, and 48 of the 118 RhC(+) samples were positive for both anti-D adsorption/elution test and SSP-PCR technique for RHD 1227A allele.
The sensitivity and specificity were 96% and 100%, respectively, for RHD 1227A detection as compared with the adsorption/elution test.
In conclusion, RhC(+) phenotype can combine with anti-D adsorption/elution test and RHD 1227A allele SSP-PCR technique for distinguishing true RhD-negative from DEL phenotype.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Srijinda, Songsak& Suwanasophon, Chamaiporn& Visawapoka, Unchalee& Pongsavee, Malinee. 2012. RhC Phenotyping, AdsorptionElution Test, and SSP-PCR : The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors. ISRN Hematology،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-465611
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Srijinda, Songsak…[et al.]. RhC Phenotyping, AdsorptionElution Test, and SSP-PCR : The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors. ISRN Hematology No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-465611
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Srijinda, Songsak& Suwanasophon, Chamaiporn& Visawapoka, Unchalee& Pongsavee, Malinee. RhC Phenotyping, AdsorptionElution Test, and SSP-PCR : The Combined Test for D-Elute Phenotype Screening in Thai RhD-Negative Blood Donors. ISRN Hematology. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-465611
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465611
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر