Fluorescence and Photochemical Investigations of Phytochrome in Higher Plants

الملخص EN

In higher plants, photoreceptor phytochrome (phy)—photoisomerizing biliprotein working as a light-driven molecular switch—is represented by a small family of phytochrome gene products with phyA and phyB as major species.

phyA is unique among other phytochromes mediating photoresponse modes specific only for this pigment (far-red light induced) and also photoresponses characteristic of phyB and other minor phys (red light induced).

In our group, in vivo fluorescence investigations of phytochrome were initiated and two native phyA pools—posttranslationally modified PHYA gene products designated phyA′ and phyA″—were detected in dicots and monocots.

They differ by spectroscopic and photochemical parameters, by abundance and distribution in etiolated plant tissues, by light stability, and other phenomenological characteristics, and, most importantly, by their functional properties.

This may explain, at least partially, the nature of the uniqueness of the phyA action.

In this paper, the data on the phyA polymorphism are summarized with attention to the applied experimental approach.