Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation
المؤلفون المشاركون
Hsu, Jia-Wei
Juan, Hsueh-Fen
Chen, Shui-Tein
Huang, Hsuan-Cheng
Wong, Chi-Huey
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-01-04
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Differentiation therapy by induction of tumor cells is an important method in the treatment of hematological cancers such as leukemia.
Tumor cell differentiation ends cancer cells' immortality, thus stopping cell growth and proliferation.
In our previous study, we found that fucose-containing polysaccharide fraction F3 extracted from Ganoderma lucidum can bring about cytokine secretion and cell death in human leukemia THP-1 cells.
This prompted us to further investigate on how F3 induces the differentiation in human leukemia cells.
We integrated time-course microarray analysis and network modeling to study the F3-induced effects on THP-1 cells.
In addition, we determined the differentiation effect using Liu's staining, nitroblue tetrazolium (NBT) reduction assay, flow cytometer, western blotting and Q-PCR.
We also examined the modulation and regulation by F3 during the differentiation process.
Dynamic gene expression profiles showed that cell differentiation was induced in F3-treated THP-1 cells.
Furthermore, F3-treated THP-1 cells exhibited enhanced macrophage differentiation, as demonstrated by changes in cell adherence, cell cycle arrest, NBT reduction and expression of differentiation markers including CD11b, CD14, CD68, matrix metalloproteinase-9 and myeloperoxidase.
In addition, caspase cleavage and p53 activation were found to be significantly enhanced in F3-treated THP-1 cells.
We unraveled the role of caspases and p53 in F3-induced THP-1 cells differentiation into macrophages.
Our results provide a molecular explanation for the differentiation effect of F3 on human leukemia THP-1 cells and offer a prospect for a potential leukemia differentiation therapy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Hsu, Jia-Wei& Huang, Hsuan-Cheng& Chen, Shui-Tein& Wong, Chi-Huey& Juan, Hsueh-Fen. 2011. Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-465650
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Hsu, Jia-Wei…[et al.]. Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-13.
https://search.emarefa.net/detail/BIM-465650
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Hsu, Jia-Wei& Huang, Hsuan-Cheng& Chen, Shui-Tein& Wong, Chi-Huey& Juan, Hsueh-Fen. Ganoderma lucidum Polysaccharides Induce Macrophage-Like Differentiation in Human Leukemia THP-1 Cells via Caspase and p53 Activation. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-13.
https://search.emarefa.net/detail/BIM-465650
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465650
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر