Local Assemblies of Paired-End Reduced Representation Libraries Sequenced with the Illumina Genome Analyzer in Maize
المؤلفون المشاركون
Zhang, Yun
Deschamps, Stéphane
Hayes, Kevin
Nannapaneni, Kishore
المصدر
International Journal of Plant Genomics
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-10-09
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The use of next-generation DNA sequencing technologies has greatly facilitated reference-guided variant detection in complex plant genomes.
However, complications may arise when regions adjacent to a read of interest are used for marker assay development, or when reference sequences are incomplete, as short reads alone may not be long enough to ascertain their uniqueness.
Here, the possibility of generating longer sequences in discrete regions of the large and complex genome of maize is demonstrated, using a modified version of a paired-end RAD library construction strategy.
Reads are generated from DNA fragments first digested with a methylation-sensitive restriction endonuclease, sheared, enriched with biotin and a selective PCR amplification step, and then sequenced at both ends.
Sequences are locally assembled into contigs by subgrouping pairs based on the identity of the read anchored by the restriction site.
This strategy applied to two maize inbred lines (B14 and B73) generated 183,609 and 129,018 contigs, respectively, out of which at least 76% were >200 bps in length.
A subset of putative single nucleotide polymorphisms from contigs aligning to the B73 reference genome with at least one mismatch was resequenced, and 90% of those in B14 were confirmed, indicating that this method is a potent approach for variant detection and marker development in species with complex genomes or lacking extensive reference sequences.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Deschamps, Stéphane& Nannapaneni, Kishore& Zhang, Yun& Hayes, Kevin. 2012. Local Assemblies of Paired-End Reduced Representation Libraries Sequenced with the Illumina Genome Analyzer in Maize. International Journal of Plant Genomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465819
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Deschamps, Stéphane…[et al.]. Local Assemblies of Paired-End Reduced Representation Libraries Sequenced with the Illumina Genome Analyzer in Maize. International Journal of Plant Genomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-465819
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Deschamps, Stéphane& Nannapaneni, Kishore& Zhang, Yun& Hayes, Kevin. Local Assemblies of Paired-End Reduced Representation Libraries Sequenced with the Illumina Genome Analyzer in Maize. International Journal of Plant Genomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-465819
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465819
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر