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Laccase-Based CLEAs : Chitosan as a Novel Cross-Linking Agent
المؤلفون المشاركون
Arsenault, Alexandre
Cabana, Hubert
Jones, J. Peter
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-07-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Laccase from Coriolopsis Polyzona was insolubilized as cross-linked enzyme aggregates (CLEAs) for the first time with chitosan as the cross-linking agent.
Concentrations between 0.01 and 1.867 g/L of chitosan were used and between 0.05 and 600 mM of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride.
The laccase was precipitated using ammonium sulphate and cross-linked simultaneously.
Specific activity and thermal stability of these biocatalysts were measured.
Activities of up to 737 U/g were obtained when 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) was used as a substrate.
Moreover, the stability of these biocatalysts was improved with regards to thermal degradation compared to free laccase when exposed to denaturing conditions of high temperature and low pH.
The CLEAs stability against chemical denaturants was also tested but no significant improvement was detected.
The total amount of ABTS to be oxidized during thermal degradation by CLEAs and free laccase was calculated and the insolubilized enzymes were reported to oxidize more substrate than free laccase.
The formation conditions were analyzed by response surface methodology in order to determine an optimal environment for the production of efficient laccase-based CLEAs using chitosan as the cross-linking agent.
After 24 hours of formation at pH 3 and at 4°C without agitation, the CLEAs exhibit the best specific activity.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Arsenault, Alexandre& Cabana, Hubert& Jones, J. Peter. 2011. Laccase-Based CLEAs : Chitosan as a Novel Cross-Linking Agent. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-467190
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Arsenault, Alexandre…[et al.]. Laccase-Based CLEAs : Chitosan as a Novel Cross-Linking Agent. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-467190
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Arsenault, Alexandre& Cabana, Hubert& Jones, J. Peter. Laccase-Based CLEAs : Chitosan as a Novel Cross-Linking Agent. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-467190
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467190
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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