Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
المؤلفون المشاركون
Irwin, Michael G.
Zhang, Liangqing
Feng, Du
Gu, Xiaoxia
Cai, Shuyun
Chen, Zhuo
Zhuang, Haixia
Hu, Zhe
Lu, Zhiqi
Xia, Zhengyuan
Ruan, Zhihua
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-22
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Recent studies indicate that propofol may protect cells via suppressing autophagic cell death caused by excessive reactive oxygen species induced by hypoxia reoxygenation (H/R).
It is established that gene expression patterns including autophagy-related genes changed significantly during the process of H/R in the presence or absence of propofol posthypoxia treatment (P-PostH).
The reasons for such differences, however, remain largely unknown.
MicroRNAs provide a novel mechanism for gene regulation.
In the present study, we systematically analyzed the alterations in microRNA expression using human umbilical vein endothelial cells (HUVECs) subjected to H/R in the presence or absence of posthypoxic propofol treatment.
Genome-wide profiling of microRNAs was then conducted using microRNA microarray.
Fourteen miRNAs are differentially expressed and six of them were validated by the quantitative real-time PCR (Q-PCR) of which three were substantially increased, whereas one was decreased.
To gain an unbiased global perspective on subsequent regulation by altered miRNAs, predicted targets of ten miRNAs were analyzed using the Gene Ontology (GO) analysis to build signaling networks.
Interestingly, six of the identified microRNAs are known to target autophagy-related genes.
In conclusion, our results revealed that different miRNA expression patterns are induced by propofol posthypoxia treatment in H/R and the alterations in miRNA expression patterns are implicated in regulating distinctive autophagy-related gene expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Zhuo& Hu, Zhe& Lu, Zhiqi& Cai, Shuyun& Gu, Xiaoxia& Zhuang, Haixia…[et al.]. 2013. Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467325
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Zhuo…[et al.]. Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-467325
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Zhuo& Hu, Zhe& Lu, Zhiqi& Cai, Shuyun& Gu, Xiaoxia& Zhuang, Haixia…[et al.]. Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467325
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467325
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر