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Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins : Studies on Specificity and Cytotoxicity
المؤلفون المشاركون
Timonen, Oskari
Lesch, Hanna P.
Ylä-Herttuala, Seppo
Schenkwein, Diana
Turkki, Vesa
Husso, Tiia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-20
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Rare-cutting endonucleases, such as the I-PpoI, can be used for the induction of double strand breaks (DSBs) in genome editing and targeted integration based on homologous recombination.
For therapeutic approaches, the specificity and the pattern of off-target effects are of high importance in these techniques.
For its applications, the endonuclease needs to be transported into the target cell nucleus, where the mechanism of transport may affect its function.
Here, we have studied the lentiviral protein transduction of the integrase (IN)-PpoI fusion protein using the cis-packaging method.
In genome-wide interaction studies, IN-fusion proteins were verified to bind their target sequence containing 28S ribosomal RNA (rRNA) genes with a 100-fold enrichment, despite the well-documented behavior of IN to be tethered into various genomic areas by host-cell factors.
In addition, to estimate the applicability of the method, DSB-induced cytotoxic effects with different vector endonuclease configurations were studied in a panel of cells.
Varying the amount and activity of endonuclease enabled the adjustment of ratio between the induced DSBs and transported DNA.
In cell studies, certain cancerous cell lines were especially prone to DSBs in rRNA genes, which led us to test the protein transduction in a tumour environment in an in vivo study.
In summary, the results highlight the potential of lentiviral vectors (LVVs) for the nuclear delivery of endonucleases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Turkki, Vesa& Schenkwein, Diana& Timonen, Oskari& Husso, Tiia& Lesch, Hanna P.& Ylä-Herttuala, Seppo. 2014. Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins : Studies on Specificity and Cytotoxicity. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-467400
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Turkki, Vesa…[et al.]. Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins : Studies on Specificity and Cytotoxicity. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-467400
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Turkki, Vesa& Schenkwein, Diana& Timonen, Oskari& Husso, Tiia& Lesch, Hanna P.& Ylä-Herttuala, Seppo. Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins : Studies on Specificity and Cytotoxicity. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-467400
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467400
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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