Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient Mesenchymal Tissue
المؤلفون المشاركون
Rajendran, Simon
Collins, Sara A.
van Pijkeren, Jan P.
Casey, Garrett
Morrissey, David
Tangney, Mark
O'Sullivan, Gerald C.
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-07-01
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Purpose.
In vivo gene therapy directed at tissues of mesenchymal origin could potentially augment healing.
We aimed to assess the duration and magnitude of transene expression in vivo in mice and ex vivo in human tissues.
Methods.
Using bioluminescence imaging, plasmid and adenoviral vector-based transgene expression in murine quadriceps in vivo was examined.
Temporal control was assessed using a doxycycline-inducible system.
An ex vivo model was developed and optimised using murine tissue, and applied in ex vivo human tissue.
Results.
In vivo plasmid-based transgene expression did not silence in murine muscle, unlike in liver.
Although maximum luciferase expression was higher in muscle with adenoviral delivery compared with plasmid, expression reduced over time.
The inducible promoter cassette successfully regulated gene expression with maximum levels a factor of 11 greater than baseline.
Expression was re-induced to a similar level on a temporal basis.
Luciferase expression was readily detected ex vivo in human muscle and tendon.
Conclusions.
Plasmid constructs resulted in long-term in vivo gene expression in skeletal muscle, in a controllable fashion utilising an inducible promoter in combination with oral agents.
Successful plasmid gene transfection in human ex vivo mesenchymal tissue was demonstrated for the first time.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Morrissey, David& van Pijkeren, Jan P.& Rajendran, Simon& Collins, Sara A.& Casey, Garrett& O'Sullivan, Gerald C.…[et al.]. 2012. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient Mesenchymal Tissue. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-467450
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Morrissey, David…[et al.]. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient Mesenchymal Tissue. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-467450
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Morrissey, David& van Pijkeren, Jan P.& Rajendran, Simon& Collins, Sara A.& Casey, Garrett& O'Sullivan, Gerald C.…[et al.]. Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient Mesenchymal Tissue. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-467450
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467450
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر