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Leaf Extracts of Calocedrus formosana (Florin) Induce G2M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells
المؤلفون المشاركون
Cheng, Ya-Wen
Hsu, Shih-Lan
Cheng, Chen-Li
Lin, Chi-Chen
Yuan, Sheau-Yun
Yang, Chi-Rei
Wu, Jyh-Horng
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-06-12
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Calocedrus formosana (Florin) bark acetone/ethylacetate extracts are known to exert an antitumor effect on some human cancer cell lines, but the mechanism is yet to be defined.
The aim of this study was to determine the effects of Florin leaf methanol extracts on the growth and apoptosis of human bladder cancer cell lines.
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay showed that the growth of these bladder cancer cells was potently inhibited by the Florin leaf extracts.
The cell cycle of these extract-treated cells (TCCSUP cells) was arrested at the G2/M phase as determined by flow cytometry.
Western blot analysis revealed the increases of cyclin B1 and Cdc2 kinase levels, alone with the decrease of phosphorylated Cdc2 kinase, after treating these cells with the extracts.
An immunofluorescence assessment of β-tubulin showed decreased levels of polymerized tubulin in treated cells.
However, the proteolytic cleavage of poly ADP-ribose polymerase and the activation of caspase-3/-8/-9 were all increased upon treatments of extracts.
The concurrent increase of Bax and decrease of Bcl-2 levels indicated that the extracts could induce apoptosis in these treated cells.
Taken together, these results suggest that the Florin leaf extracts may be an effective antibladder cancer agent.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Yuan, Sheau-Yun& Lin, Chi-Chen& Hsu, Shih-Lan& Cheng, Ya-Wen& Wu, Jyh-Horng& Cheng, Chen-Li…[et al.]. 2011. Leaf Extracts of Calocedrus formosana (Florin) Induce G2M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-467533
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Yuan, Sheau-Yun…[et al.]. Leaf Extracts of Calocedrus formosana (Florin) Induce G2M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-467533
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Yuan, Sheau-Yun& Lin, Chi-Chen& Hsu, Shih-Lan& Cheng, Ya-Wen& Wu, Jyh-Horng& Cheng, Chen-Li…[et al.]. Leaf Extracts of Calocedrus formosana (Florin) Induce G2M Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-467533
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467533
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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