![](/images/graphics-bg.png)
Proteomic Analysis of Plasma-Purified VLDL, LDL, and HDL Fractions from Atherosclerotic Patients Undergoing Carotid Endarterectomy : Identification of Serum Amyloid A as a Potential Marker
المؤلفون المشاركون
Carta, Franco
Nieddu, Gabriele
Piredda, Franco
Formato, Marilena
Zinellu, Elisabetta
Turrini, Francesco
Spirito, Rita
De Muro, Pierina
Guarino, Anna
Lepedda, Antonio Junior
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-12-24
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
Apolipoproteins are very heterogeneous protein family, implicated in plasma lipoprotein structural stabilization, lipid metabolism, inflammation, or immunity.
Obtaining detailed information on apolipoprotein composition and structure may contribute to elucidating lipoprotein roles in atherogenesis and to developing new therapeutic strategies for the treatment of lipoprotein-associated disorders.
This study aimed at developing a comprehensive method for characterizing the apolipoprotein component of plasma VLDL, LDL, and HDL fractions from patients undergoing carotid endarterectomy, by means of two-dimensional electrophoresis (2-DE) coupled with Mass Spectrometry analysis, useful for identifying potential markers of plaque presence and vulnerability.
The adopted method allowed obtaining reproducible 2-DE maps of exchangeable apolipoproteins from VLDL, LDL, and HDL.
Twenty-three protein isoforms were identified by peptide mass fingerprinting analysis.
Differential proteomic analysis allowed for identifying increased levels of acute-phase serum amyloid A protein (AP SAA) in all lipoprotein fractions, especially in LDL from atherosclerotic patients.
Results have been confirmed by western blotting analysis on each lipoprotein fraction using apo AI levels for data normalization.
The higher levels of AP SAA found in patients suggest a role of LDL as AP SAA carrier into the subendothelial space of artery wall, where AP SAA accumulates and may exert noxious effects.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lepedda, Antonio Junior& Nieddu, Gabriele& Zinellu, Elisabetta& De Muro, Pierina& Piredda, Franco& Guarino, Anna…[et al.]. 2013. Proteomic Analysis of Plasma-Purified VLDL, LDL, and HDL Fractions from Atherosclerotic Patients Undergoing Carotid Endarterectomy : Identification of Serum Amyloid A as a Potential Marker. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467913
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lepedda, Antonio Junior…[et al.]. Proteomic Analysis of Plasma-Purified VLDL, LDL, and HDL Fractions from Atherosclerotic Patients Undergoing Carotid Endarterectomy : Identification of Serum Amyloid A as a Potential Marker. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-467913
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lepedda, Antonio Junior& Nieddu, Gabriele& Zinellu, Elisabetta& De Muro, Pierina& Piredda, Franco& Guarino, Anna…[et al.]. Proteomic Analysis of Plasma-Purified VLDL, LDL, and HDL Fractions from Atherosclerotic Patients Undergoing Carotid Endarterectomy : Identification of Serum Amyloid A as a Potential Marker. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-467913
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467913
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)