cDNA-SRAP and Its Application in Differential Gene Expression Analysis : A Case Study in Erianthus arundinaceum
المؤلفون المشاركون
You-Xiong, Que
Xu, Jingsheng
Li-Ping, Xu
Zheng, Jin
Chen, Rukai
Luo, Jun
Jian-Wei, Lin
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-19
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Erianthus arundinaceum is a wild relative species of sugarcane.
The aim of this research was to demonstrate the feasibility of cDNA-SRAP for differential gene expression and to explore the molecular mechanism of drought resistance in E.
arundinaceum.
cDNA-SRAP technique, for the first time, was applied in the analysis of differential gene expression in E.
arundinaceum under drought stress.
In total, eight differentially expressed genes with length of 185–427 bp were successfully isolated (GenBank Accession numbers: EU071770, EU071772, EU071774, EU071776, EU071777, EU071779, EU071780, and EU071781).
Based on their homologies with genes in GenBank, these genes were assumed to encode ribonuclease III, vacuolar protein, ethylene insensitive protein, aerobactin biosynthesis protein, photosystem II protein, glucose transporter, leucine-rich repeat protein, and ammonia monooxygenase.
Real-time PCR analysis on the expression profiling of gene (EU071774) encoding ethylene-insensitive protein and gene (EU071781) encoding ammonia monooxygenase revealed that the expression of these two genes was upregulated both by PEG and ABA treatments, suggesting that they may involve in the drought resistance of E.
arundinaceum.
This study constitutes the first report of genes activated in E.
arundinaceum by drought stress and opens up the application of cDNA-SRAP in differential gene expression analysis in E.
arundinaceum under certain stress conditions.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
You-Xiong, Que& Li-Ping, Xu& Luo, Jun& Xu, Jingsheng& Zheng, Jin& Chen, Rukai…[et al.]. 2012. cDNA-SRAP and Its Application in Differential Gene Expression Analysis : A Case Study in Erianthus arundinaceum. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-468292
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Jun…[et al.]. cDNA-SRAP and Its Application in Differential Gene Expression Analysis : A Case Study in Erianthus arundinaceum. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-468292
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
You-Xiong, Que& Li-Ping, Xu& Luo, Jun& Xu, Jingsheng& Zheng, Jin& Chen, Rukai…[et al.]. cDNA-SRAP and Its Application in Differential Gene Expression Analysis : A Case Study in Erianthus arundinaceum. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-468292
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-468292
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر