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Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity
المؤلفون المشاركون
Sadeghein, Bahareh
Moghadam, Nazanin
Yaghmaei, Soheila
Ghobadi Nejad, Zahra
المصدر
International Journal of Chemical Engineering
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-19
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
العلوم الهندسية و تكنولوجيا المعلومات
الملخص EN
Due to great commercial application of protease, it is necessary to study kinetic characterization of this enzyme in order to improve design of enzymatic reactors.
In this study, mathematical modeling of protease enzyme production kinetics which is derived from Bacillus licheniformis BBRC 100053 was studied (at 37°C, pH 10 after 73 h in stationary phase, and 150 rpm).
The aim of the present paper was to determine the best kinetic model and kinetic parameters for production of protease and calculating Ki (inhibition constant) of different inhibitors to find the most effective one.
The kinetic parameters Km (Michaelis-Menten constant) and Vm (maximum rate) were calculated 0.626 mM and 0.0523 mM/min.
According to the experimental results, using DFP (diisopropyl fluorophosphate) and PMSF (phenylmethanesulfonyl fluoride) as inhibitors almost 50% of the enzyme activity could be inhibited when their concentrations were 0.525 and 0.541 mM, respectively.
Ki for DFP and PMSF were 0.46 and 0.56 mM, respectively.
Kinetic analysis showed that the Lineweaver-Burk model was the best fitting model for protease production kinetics DFP was more effective than PMSF and both of them should be covered in the group of noncompetitive inhibitors.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ghobadi Nejad, Zahra& Yaghmaei, Soheila& Moghadam, Nazanin& Sadeghein, Bahareh. 2014. Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity. International Journal of Chemical Engineering،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-468719
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ghobadi Nejad, Zahra…[et al.]. Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity. International Journal of Chemical Engineering No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-468719
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ghobadi Nejad, Zahra& Yaghmaei, Soheila& Moghadam, Nazanin& Sadeghein, Bahareh. Some Investigations on Protease Enzyme Production Kinetics Using Bacillus licheniformis BBRC 100053 and Effects of Inhibitors on Protease Activity. International Journal of Chemical Engineering. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-468719
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-468719
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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