![](/images/graphics-bg.png)
Sequence and Apoptotic Activity of VacA Cytotoxin Cloned from a Helicobacter pylori Thai Clinical Isolate
المؤلفون المشاركون
Yousef, Muhammad
Junaid, Muhammad
Leetachewa, Somphob
Angsuthanasombat, Chanan
Muanprasat, Chatchai
Katzenmeier, Gerd
Ali, Niaz
Chaicumpa, Wanpen
al-Gubare, Sarbast
Na Ubol, Mathukorn
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-02
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The vacuolating cytotoxin VacA produced by Helicobacter pylori induces the formation of large cytoplasmic vacuoles in host gastric epithelial cells as well as a release of cytochrome C from mitochondria resulting in cell apoptosis.
Considerable sequence diversity in VacA relating to different degrees of disease severity is observed with clinical samples from a multitude of geographic places.
In this study we describe expression in Escherichia coli, purification to homogeneity and in vitro assay of its apoptotic activity of a VacA toxin from a H.
pylori isolate of a Thai patient with gastrointestinal lymphoma.
Sequencing revealed that the deduced amino acid sequence of the cloned Thai isolate VacA is similar to H.
pylori s1/m2 type strains.
The percent sequence similarity to the model strain 60190 was lower due to the presence of extra amino acids in the mid (m) region.
The purified VacA toxin exhibited significant apoptotic activity on both T84 and MDCK epithelial cell lines, as revealed by DAPI staining, whereby the observed activity was significantly higher on MDCK cells.
These findings could relate to a modulation of VacA activity on host cells in the Thai isolate-VacA toxin that may differ from those of the model strain.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Junaid, Muhammad& al-Gubare, Sarbast& Yousef, Muhammad& Na Ubol, Mathukorn& Leetachewa, Somphob& Muanprasat, Chatchai…[et al.]. 2014. Sequence and Apoptotic Activity of VacA Cytotoxin Cloned from a Helicobacter pylori Thai Clinical Isolate. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-469010
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Junaid, Muhammad…[et al.]. Sequence and Apoptotic Activity of VacA Cytotoxin Cloned from a Helicobacter pylori Thai Clinical Isolate. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-469010
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Junaid, Muhammad& al-Gubare, Sarbast& Yousef, Muhammad& Na Ubol, Mathukorn& Leetachewa, Somphob& Muanprasat, Chatchai…[et al.]. Sequence and Apoptotic Activity of VacA Cytotoxin Cloned from a Helicobacter pylori Thai Clinical Isolate. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-469010
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-469010
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)