Topography of Lipid Droplet-Associated Proteins : Insights from Freeze-Fracture Replica Immunogold Labeling

المؤلفون المشاركون

Robenek, Mirko J.
Ruebel, Anneke
Hofnagel, Oliver
Troyer, David
Severs, Nicholas J.
Buers, Insa
Robenek, Horst

المصدر

Journal of Lipids

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-12-26

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الطب البشري
علوم

الملخص EN

Lipid droplets are not merely storage depots for superfluous intracellular lipids in times of hyperlipidemic stress, but metabolically active organelles involved in cellular homeostasis.

Our concepts on the metabolic functions of lipid droplets have come from studies on lipid droplet-associated proteins.

This realization has made the study of proteins, such as PAT family proteins, caveolins, and several others that are targeted to lipid droplets, an intriguing and rapidly developing area of intensive inquiry.

Our existing understanding of the structure, protein organization, and biogenesis of the lipid droplet has relied heavily on microscopical techniques that lack resolution and the ability to preserve native cellular and protein composition.

Freeze-fracture replica immunogold labeling overcomes these disadvantages and can be used to define at high resolution the precise location of lipid droplet-associated proteins.

In this paper illustrative examples of how freeze-fracture immunocytochemistry has contributed to our understanding of the spatial organization in the membrane plane and function of PAT family proteins and caveolin-1 are presented.

By revisiting the lipid droplet with freeze-fracture immunocytochemistry, new perspectives have emerged which challenge prevailing concepts of lipid droplet biology and may hopefully provide a timely impulse for many ongoing studies.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Robenek, Horst& Buers, Insa& Robenek, Mirko J.& Hofnagel, Oliver& Ruebel, Anneke& Troyer, David…[et al.]. 2010. Topography of Lipid Droplet-Associated Proteins : Insights from Freeze-Fracture Replica Immunogold Labeling. Journal of Lipids،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-469875

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Robenek, Horst…[et al.]. Topography of Lipid Droplet-Associated Proteins : Insights from Freeze-Fracture Replica Immunogold Labeling. Journal of Lipids No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-469875

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Robenek, Horst& Buers, Insa& Robenek, Mirko J.& Hofnagel, Oliver& Ruebel, Anneke& Troyer, David…[et al.]. Topography of Lipid Droplet-Associated Proteins : Insights from Freeze-Fracture Replica Immunogold Labeling. Journal of Lipids. 2010. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-469875

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-469875