Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells
المؤلفون المشاركون
Martino, Sabata
Orlacchio, Aldo
D'Angelo, Francesco
di Girolamo, Ilaria
Tiribuzi, Roberto
Datti, Alessandro
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-05-31
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
RNA interference technology is an ideal strategy to elucidate the mechanisms associated with human CD34+ hematopoietic stem cell differentiation into dendritic cells.
Simple manipulations in vitro can unequivocally yield alloreactive or tolerogenic populations, suggesting key implications of biochemical players that might emerge as therapeutic targets for cancer or graft-versus-host disease.
To knockdown proteins typically involved in the biology of dendritic cells, we employed an siRNA delivery system based on the cationic liposome DOTAP as the carrier.
Freshly-isolated CD34+ cells were transfected with siRNA for cathepsin S with negligible cytotoxicity and transfection rates (>60%) comparable to the efficiency shown by lentiviral vectors.
Further, cathepsin S knockdown was performed during both cell commitment and through the entire 14-day differentiation process with repeated transfection rounds that had no effect per se on cell development.
Tested in parallel, other commercially-available chemical reagents failed to meet acceptable standards.
In addition to safe and practical handling, a direct advantage of DOTAP over viral-mediated techniques is that transient silencing effects can be dynamically appraised through the recovery of targeted proteins.
Thus, our findings identify DOTAP as an excellent reagent for gene silencing in resting and differentiating CD34+ cells, suggesting a potential for applications in related preclinical models.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Martino, Sabata& di Girolamo, Ilaria& Tiribuzi, Roberto& D'Angelo, Francesco& Datti, Alessandro& Orlacchio, Aldo. 2009. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-469937
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Martino, Sabata…[et al.]. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-469937
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Martino, Sabata& di Girolamo, Ilaria& Tiribuzi, Roberto& D'Angelo, Francesco& Datti, Alessandro& Orlacchio, Aldo. Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-469937
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-469937
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر