![](/images/graphics-bg.png)
Primary Genetic Investigation of a Hyperlipidemia Model : Molecular Characteristics and Variants of the Apolipoprotein E Gene in Mongolian Gerbil
المؤلفون المشاركون
Guo, Honggang
Ying, Huazhong
Liu, Yuehuan
Wu, Jiusheng
Shi, Qiaojuan
Xu, Ningying
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-29
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The objective of this work was to establish a novel Mongolian gerbil (Meriones unguiculatus) hyperlipidemia model and to investigate its susceptibility genetic basis.
Two rodent (gerbil and rat) hyperlipidemia models were induced by feeding a high fat/high-cholesterol (HF/HC) diet.
There were significant increases of serum total cholesterol, triglycerides, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) in gerbils within a 4-week modeling period.
About 10–30% of >8-month-old individuals developed hyperlipidemia spontaneously.
The apolipoprotein E (ApoE) gene was cloned by merging a sequence of rapid amplification of cDNA ends (RACE) and nested polymerase chain reaction products.
The results revealed an open reading frame of 948 bp, encoding a protein of 298 amino acids.
The gene without a 5′-UTR region in the first intron was highly homologous to human Apo-A-I and rat Apo-A-IV.
The distribution of expression of the ApoE gene in liver, brain, heart, lung, kidney, and adrenal gland was detected by an ABC immunohistochemical procedure.
Three single nucleotide polymorphisms (SNPs; C97T, G781T, and A1774T) were first found using PCR-single-strand conformation polymorphism (PCR-SSCP) in a closed population containing 444 animals.
Correlation analysis confirmed that new SNPs , age, and gender were associated significantly (P<0.05) with hyperlipidemia.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yuehuan& Wu, Jiusheng& Shi, Qiaojuan& Guo, Honggang& Ying, Huazhong& Xu, Ningying. 2014. Primary Genetic Investigation of a Hyperlipidemia Model : Molecular Characteristics and Variants of the Apolipoprotein E Gene in Mongolian Gerbil. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-469965
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yuehuan…[et al.]. Primary Genetic Investigation of a Hyperlipidemia Model : Molecular Characteristics and Variants of the Apolipoprotein E Gene in Mongolian Gerbil. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-469965
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yuehuan& Wu, Jiusheng& Shi, Qiaojuan& Guo, Honggang& Ying, Huazhong& Xu, Ningying. Primary Genetic Investigation of a Hyperlipidemia Model : Molecular Characteristics and Variants of the Apolipoprotein E Gene in Mongolian Gerbil. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-469965
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-469965
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
![](/images/ebook-kashef.png)
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
![](/images/kashef-image.png)