Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates
المؤلفون المشاركون
Chung, Jing-Gung
Li, Mi
Ho, Heng-Chien
Liu, Yu-Ching
Lee, Miau-Rong
Chen, Chao-Jung
Hsieh, Wen-Tsong
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-24
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Numerous reports illustrate the diverse effects of chewing the areca nut, most of which are harmful and have been shown to be associated with oral cancer.
Nearly all of the studies are focused on the extract and/or low molecular weight ingredients in the areca nut.
The purpose of this report is to identify the major protein component in the areca nut.
After ammonium sulfate fractionation, the concentrated areca nut extract is subjected to DEAE-cellulose chromatography.
A colored protein is eluted at low NaCl concentration and the apparently homogeneous eluent represents the major protein component compared to the areca nut extract.
The colored protein shares partial sequence identity with the royal palm tree peroxidase and its peroxidase activity is confirmed using an established assay.
In the study, the natural substrates of areca nut peroxidase are identified as catechin, epicatechin, and procyanidin B1.
The two former substrates are similarly oxidized to form a 576 Da product with concomitant removal of four hydrogen atoms.
Interestingly, oxidation of procyanidin B1 occurs only in the presence of catechin or epicatechin and an additional product with an 864 Da molecular mass.
In addition, procyanidin B1 is identified as a peroxidase substrate for the first time.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Yu-Ching& Chen, Chao-Jung& Lee, Miau-Rong& Li, Mi& Hsieh, Wen-Tsong& Chung, Jing-Gung…[et al.]. 2013. Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470114
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Yu-Ching…[et al.]. Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-12.
https://search.emarefa.net/detail/BIM-470114
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Yu-Ching& Chen, Chao-Jung& Lee, Miau-Rong& Li, Mi& Hsieh, Wen-Tsong& Chung, Jing-Gung…[et al.]. Peroxidase as the Major Protein Constituent in Areca Nut and Identification of Its Natural Substrates. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-12.
https://search.emarefa.net/detail/BIM-470114
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-470114
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر