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Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis
المؤلفون المشاركون
Yu, Ting
Li, Yanqun
Wen, Zhongyang
Cao, Wei
Zhang, Bo
Chen, Bingni
Zhou, Xiaohong
Li, Feng
Zhang, Jinshun
Ye, Liang
Li, Xiaoqing
Huang, Zhong
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-25
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
To gain further insights into the molecular basis of Sulforaphane (SF) mediated retinal pigment epithelial (RPE) 19 cell against oxidative stress, we investigated the effects of SF on the regulation of gene expression on a global scale and tested whether SF can endow RPE cells with the ability to resist apoptosis.
The data revealed that after exposure to H2O2, RPE 19 cell viability was increased in the cells pretreated with SF compared to the cell not treated with SF.
Microarray analysis revealed significant changes in the expression of 69 genes in RPE 19 cells after 6 hours of SF treatment.
Based on the functional relevance, eight of the SF-responsive genes, that belong to antioxidant redox system, and inflammatory responsive factors were validated.
The up-regulating translation of thioredoxin-1 (Trx1) and the nuclear translocation of Nuclear factor-like2 (Nrf2) were demonstrated by immunoblot analysis in SF treated RPE cells.
Our data indicate that SF increases the ability of RPE 19 cell against oxidative stress through up-regulating antioxidative enzymes and down-regulating inflammatory mediators and chemokines.
The results suggest that the antioxidant, SF, may be a valuable supplement for preventing and retarding the development of Age Related Macular Degeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ye, Liang& Yu, Ting& Li, Yanqun& Chen, Bingni& Zhang, Jinshun& Wen, Zhongyang…[et al.]. 2013. Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-470130
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ye, Liang…[et al.]. Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-470130
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ye, Liang& Yu, Ting& Li, Yanqun& Chen, Bingni& Zhang, Jinshun& Wen, Zhongyang…[et al.]. Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-470130
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-470130
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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