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Hemoculture and Polymerase Chain Reaction Using Primers TCZ1TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis
المؤلفون المشاركون
Eloy, Luciano José
Lucheis, Simone Baldini
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-05-31
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Introduction.
American trypanosomiasis, also known as Chagas disease, is a zoonosis caused by Trypanosoma cruzi (T.
cruzi).
Dogs and cats participate actively in this parasite's transmission cycle.
This study aimed at evaluating the occurrence of T.
cruzi in dogs and cats from Botucatu, SP, Brazil, as well as at evaluating the technique of hemoculture in LIT (liver infusion tryptose) medium by polymerase chain reaction (PCR).
Methods.
Blood samples were collected from 50 dogs and 50 cats in Botucatu-SP, Brazil.
For hemoculture, the samples were inoculated in LIT medium, and readings were performed for four months.
Upon completion of such period, all the hemocultures were processed for parasitic DNA extraction.
The PCR reactions were performed by using primers TCZ1/TCZ2.
Results.
Ten dogs and ten cats (20%) were positive to PCR, and four dogs and three cats (7%) were positive to hemoculture.
Only in a one cat sample (1%) there was confirmation of positive hemoculture by PCR for T.
cruzi.
Conclusions.
Results showed that PCR was a suitable tool for the confirmation of the parasite detection in hemoculture samples, and that dogs and cats from Botucatu, SP, Brazil, are maintaining the role of household reservoirs of T.
cruzi, which reinforces the need for constant epidemiologic surveillance for this zoonosis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Eloy, Luciano José& Lucheis, Simone Baldini. 2012. Hemoculture and Polymerase Chain Reaction Using Primers TCZ1TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis. ISRN Veterinary Science،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470735
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Eloy, Luciano José& Lucheis, Simone Baldini. Hemoculture and Polymerase Chain Reaction Using Primers TCZ1TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis. ISRN Veterinary Science No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-470735
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Eloy, Luciano José& Lucheis, Simone Baldini. Hemoculture and Polymerase Chain Reaction Using Primers TCZ1TCZ2 for the Diagnosis of Canine and Feline Trypanosomiasis. ISRN Veterinary Science. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-470735
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-470735
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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