α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism
المؤلفون المشاركون
Calla-Choque, Jaeson Santos
Figueroa-Angulo, Elisa Elvira
Arroyo, Rossana
Ávila-González, Leticia
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-20، 20ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-03-01
دولة النشر
مصر
عدد الصفحات
20
التخصصات الرئيسية
الملخص EN
Trichomonas vaginalis is a sexually transmitted flagellated protist parasite responsible for trichomoniasis.
This parasite is dependent on high levels of iron, favoring its growth and multiplication.
Iron also differentially regulates some trichomonad virulence properties by unknown mechanisms.
However, there is evidence to support the existence of gene regulatory mechanisms at the transcriptional and posttranscriptional levels that are mediated by iron concentration in T.
vaginalis.
Thus, the goal of this study was to identify an RNA-binding protein in T.
vaginalis that interacts with the tvcp4 RNA stem-loop structure, which may participate in a posttranscriptional iron regulatory mechanism mediated by RNA-protein interactions.
We performed RNA electrophoretic mobility shift assay (REMSA) and supershift, UV cross-linking, Northwestern blot, and western blot (WB) assays using cytoplasmic protein extracts from T.
vaginalis with the tvcp4 RNA hairpin structure as a probe.
We identified a 135-kDa protein isolated by the UV cross-linking assays as α-actinin 3 (TvACTN3) by MALDI-TOF-MS that was confirmed by LS-MS/MS and de novo sequencing.
TvACTN3 is a cytoplasmic protein that specifically binds to hairpin RNA structures from trichomonads and humans when the parasites are grown under iron-depleted conditions.
Thus, TvACTN3 could participate in the regulation of gene expression by iron in T.
vaginalis through a parallel posttranscriptional mechanism similar to that of the IRE/IRP system.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Calla-Choque, Jaeson Santos& Figueroa-Angulo, Elisa Elvira& Ávila-González, Leticia& Arroyo, Rossana. 2014. α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism. BioMed Research International،Vol. 2014, no. 2014, pp.1-20.
https://search.emarefa.net/detail/BIM-471121
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Calla-Choque, Jaeson Santos…[et al.]. α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism. BioMed Research International No. 2014 (2014), pp.1-20.
https://search.emarefa.net/detail/BIM-471121
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Calla-Choque, Jaeson Santos& Figueroa-Angulo, Elisa Elvira& Ávila-González, Leticia& Arroyo, Rossana. α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-20.
https://search.emarefa.net/detail/BIM-471121
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-471121
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر