Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-OnLights-Off Probes for the Detection of Pathogens Associated with Sepsis
المؤلفون المشاركون
Reis, Arthur H.
Czajka, John W.
Carver-Brown, Rachel K.
Rice, Lisa M.
Wangh, Lawrence J.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Aims.
The goal of this study was to construct a single tube molecular diagnostic multiplex assay for the detection of microbial pathogens commonly associated with septicemia, using LATE-PCR and Lights-On/Lights-Off probe technology.
Methods and Results.
The assay described here identified pathogens associated with sepsis by amplification and analysis of the 16S ribosomal DNA gene sequence for bacteria and specific gene sequences for fungi.
A sequence from an unidentified gene in Lactococcus lactis subsp.
cremoris served as a positive control for assay function.
LATE-PCR was used to generate single-stranded amplicons that were then analyzed at endpoint over a wide temperature range in a specific fluorescent color.
Each bacterial target was identified by its pattern of hybridization to Lights-On/Lights-Off probes derived from molecular beacons.
Complex mixtures of targets were also detected.
Conclusions.
All microbial targets were identified in samples containing low starting copy numbers of pathogen genomic DNA, both as individual targets and in complex mixtures.
Significance and Impact of the Study.
This assay uses new technology to achieve an advance in the field of molecular diagnostics: a single-tube multiplex assay for identification of pathogens commonly associated with sepsis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Carver-Brown, Rachel K.& Reis, Arthur H.& Rice, Lisa M.& Czajka, John W.& Wangh, Lawrence J.. 2012. Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-OnLights-Off Probes for the Detection of Pathogens Associated with Sepsis. Journal of Pathogens،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-471133
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Carver-Brown, Rachel K.…[et al.]. Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-OnLights-Off Probes for the Detection of Pathogens Associated with Sepsis. Journal of Pathogens No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-471133
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Carver-Brown, Rachel K.& Reis, Arthur H.& Rice, Lisa M.& Czajka, John W.& Wangh, Lawrence J.. Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-OnLights-Off Probes for the Detection of Pathogens Associated with Sepsis. Journal of Pathogens. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-471133
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-471133
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر