Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR
المؤلفون المشاركون
Kim, Juwon
Kim, Yoonjung
Lee, Kyung-A
Kwon, Aerin
Han, Mi-Soon
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-28
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A total of 84 nasopharyngeal swab specimens were collected from 84 patients.
Viral nucleic acid was extracted by three automated extraction systems: QIAcube (Qiagen, Germany), EZ1 Advanced XL (Qiagen), and MICROLAB Nimbus IVD (Hamilton, USA).
Fourteen RNA viruses and two DNA viruses were detected using the Anyplex II RV16 Detection kit (Seegene, Republic of Korea).
The EZ1 Advanced XL system demonstrated the best analytical sensitivity for all the three viral strains.
The nucleic acids extracted by EZ1 Advanced XL showed higher positive rates for virus detection than the others.
Meanwhile, the MICROLAB Nimbus IVD system was comprised of fully automated steps from nucleic extraction to PCR setup function that could reduce human errors.
For the nucleic acids recovered from nasopharyngeal swab specimens, the QIAcube system showed the fewest false negative results and the best concordance rate, and it may be more suitable for detecting various viruses including RNA and DNA virus strains.
Each system showed different sensitivity and specificity for detection of certain viral pathogens and demonstrated different characteristics such as turnaround time and sample capacity.
Therefore, these factors should be considered when new nucleic acid extraction systems are introduced to the laboratory.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kim, Yoonjung& Han, Mi-Soon& Kim, Juwon& Kwon, Aerin& Lee, Kyung-A. 2014. Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-471689
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kim, Yoonjung…[et al.]. Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-471689
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kim, Yoonjung& Han, Mi-Soon& Kim, Juwon& Kwon, Aerin& Lee, Kyung-A. Evaluation of Three Automated Nucleic Acid Extraction Systems for Identification of Respiratory Viruses in Clinical Specimens by Multiplex Real-Time PCR. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-471689
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-471689
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر