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Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes
المؤلفون المشاركون
Mizuki, Keiji
Kiyama, Ryoiti
Ogaeri, Takunori
Isobe, Shin-ichiro
Nishi, Kentaro
Kashiwa, Yuki
Wuxiuer, Dilibaier
Aoyagi, Tei-ichiro
Zhu, Yun
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-03
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
New fluorescent Fluolid dyes have advantages over others such as stability against heat, dryness, and excess light.
Here, we performed simultaneous immunostaining of renal tumors, clear cell renal cell carcinoma (RCC), papillary RCC, chromophobe RCC, acquired cystic disease-associated RCC (ACD-RCC), and renal angiomyolipoma (AML), with primary antibodies against Kank1, cytokeratin 7 (CK7), and CD10, which were detected with secondary antibodies labeled with Fluolid-Orange, Fluolid-Green, and Alexa Fluor 647, respectively.
Kank1 was stained in normal renal tubules, papillary RCC, and ACD-RCC, and weakly or negatively in all other tumors.
CK7 was positive in normal renal tubules, papillary RCC, and ACD-RCC.
In contrast, CD10 was expressed in renal tubules and clear cell RCC, papillary RCC, AML, and AC-RCC, and weakly in chromophobe RCC.
These results may contribute to differentiating renal tumors and subtypes of RCCs.
We also examined the stability of fluorescence and found that fluorescent images of Fluolid dyes were identical between a tissue section and the same section after it was stored for almost three years at room temperature.
This indicates that tissue sections can be stored at room temperature for a relatively long time after they are stained with multiple fluorescent markers, which could open a door for pathological diagnostics.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wuxiuer, Dilibaier& Zhu, Yun& Ogaeri, Takunori& Mizuki, Keiji& Kashiwa, Yuki& Nishi, Kentaro…[et al.]. 2014. Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-472303
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wuxiuer, Dilibaier…[et al.]. Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-472303
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wuxiuer, Dilibaier& Zhu, Yun& Ogaeri, Takunori& Mizuki, Keiji& Kashiwa, Yuki& Nishi, Kentaro…[et al.]. Development of Pathological Diagnostics of Human Kidney Cancer by Multiple Staining Using New Fluorescent Fluolid Dyes. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-472303
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-472303
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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