N-Isopropylacrylamide-co-glycidylmethacrylate as a Thermoresponsive Substrate for Corneal Endothelial Cell Sheet Engineering
المؤلفون المشاركون
Kumary, Thrikkovil Variyath
Madathil, Bernadette K.
Anil Kumar, Pallickaveedu RajanAsari
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-06-09
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Endothelial keratoplasty is a recent shift in the surgical treatment of corneal endothelial dystrophies, where the dysfunctional endothelium is replaced whilst retaining the unaffected corneal layers.
To overcome the limitation of donor corneal shortage, alternative use of tissue engineered constructs is being researched.
Tissue constructs with intact extracellular matrix are generated using stimuli responsive polymers.
In this study we evaluated the feasibility of using the thermoresponsive poly(N-isopropylacrylamide-co-glycidylmethacrylate) polymer as a culture surface to harvest viable corneal endothelial cell sheets.
Incubation below the lower critical solution temperature of the polymer allowed the detachment of the intact endothelial cell sheet.
Phase contrast and scanning electron microscopy revealed the intact architecture, cobble stone morphology, and cell-to-cell contact in the retrieved cell sheet.
Strong extracellular matrix deposition was also observed.
The RT-PCR analysis confirmed functionally active endothelial cells in the cell sheet as evidenced by the positive expression of aquaporin 1, collagen IV, Na+-K+ ATPase, and FLK-1.
Na+-K+ ATPase protein expression was also visualized by immunofluorescence staining.
These results suggest that the in-house developed thermoresponsive culture dish is a suitable substrate for the generation of intact corneal endothelial cell sheet towards transplantation for endothelial keratoplasty.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Madathil, Bernadette K.& Anil Kumar, Pallickaveedu RajanAsari& Kumary, Thrikkovil Variyath. 2014. N-Isopropylacrylamide-co-glycidylmethacrylate as a Thermoresponsive Substrate for Corneal Endothelial Cell Sheet Engineering. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472563
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Madathil, Bernadette K.…[et al.]. N-Isopropylacrylamide-co-glycidylmethacrylate as a Thermoresponsive Substrate for Corneal Endothelial Cell Sheet Engineering. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-472563
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Madathil, Bernadette K.& Anil Kumar, Pallickaveedu RajanAsari& Kumary, Thrikkovil Variyath. N-Isopropylacrylamide-co-glycidylmethacrylate as a Thermoresponsive Substrate for Corneal Endothelial Cell Sheet Engineering. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-472563
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-472563
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر