Comparison of Heavy Labeled (SIL) Peptide versus SILAC Protein Internal Standards for LC-MSMS Quantification of Hepatic Drug Transporters
المؤلفون المشاركون
Prasad, Bhagwat
Unadkat, Jashvant D.
المصدر
International Journal of Proteomics
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-25
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
We studied the precision of quantification of organic anion-transporting polypeptide 1B1 (OATP1B1), OATP1B3, OATP2B1, and P-glycoprotein (P-gp) in human livers by surrogate peptide based LC-MS/MS approach using two different internal standards: stable isotope labeled peptide (SIL) versus stable isotope labeled protein (SILAC).
The SIL peptides were procured commercially and the SILAC proteins were generated in-house by labeling arginine and/or lysine residues in cells expressing these transporters.
Liver tissue n=20 was homogenized and the membrane fraction was isolated.
The membranes were trypsin digested and the peptides were analyzed using LC-MS/MS under optimized conditions.
The precision in the quantification of proteins in three independently trypsin digested samples from each liver was calculated as the standard deviation of the log transformed protein concentration.
The precision of the SIL internal standard method was either slightly (P<0.05, paired t-test) better than that of the SILAC method (OATP1B1, OATP1B3, and P-gp) or not different (OATP2B1).
Trypsin digestion, as measured by the response of the labeled peptide derived from the SILAC protein, was consistent across liver samples.
These results indicate that when maximum trypsin digestion is ensured, the SIL internal standard method can be used with confidence for quantification of drug transporters.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Prasad, Bhagwat& Unadkat, Jashvant D.. 2014. Comparison of Heavy Labeled (SIL) Peptide versus SILAC Protein Internal Standards for LC-MSMS Quantification of Hepatic Drug Transporters. International Journal of Proteomics،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-472628
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Prasad, Bhagwat& Unadkat, Jashvant D.. Comparison of Heavy Labeled (SIL) Peptide versus SILAC Protein Internal Standards for LC-MSMS Quantification of Hepatic Drug Transporters. International Journal of Proteomics No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-472628
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Prasad, Bhagwat& Unadkat, Jashvant D.. Comparison of Heavy Labeled (SIL) Peptide versus SILAC Protein Internal Standards for LC-MSMS Quantification of Hepatic Drug Transporters. International Journal of Proteomics. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-472628
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-472628
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر