Differences in Mammalian Target of Rapamycin Gene Expression in the Peripheral Blood and Articular Cartilages of Osteoarthritic Patients and Disease Activity
المؤلفون المشاركون
Glukhova, Svetlana I.
Kashevarova, Natalya G.
Taskina, Elena A.
Tchetina, Elena V.
Zaitseva, Elena M.
Makarov, Sergey A.
Makarov, Maxim A.
Poole, A. Robin
Semyonova, Liudmila A.
Alekseeva, Liudmila I.
Kuzin, Alexandr N.
Sharapova, Eugeniya P.
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-06-25
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
The gene expression of mTOR, autophagy-related ULK1, caspase 3, CDK-inhibitor p21, and TNFα was measured in the peripheral blood of osteoarthritic (OA) patients at different stages of the disease aiming to establish a gene expression profile that might indicate the activity of the disease and joint destruction.
Whole blood of 65 OA outpatients, 27 end-stage OA patients, 27 healthy volunteers, and knee articular cartilages of 28 end-stage OA patients and 26 healthy subjects were examined.
OA outpatients were subjected to clinical testing, ultrasonography, and radiographic and WOMAC scoring.
Protein levels of p70-S6K, p21, and caspase 3 were quantified by ELISA.
Gene expression was measured using real-time RT-PCR.
Upregulation of mTOR gene expression was observed in PBMCs of 42 OA outpatients (“High mTOR expression subset”) and in PBMCs and articular cartilages of all end-stage OA patients.
A positive correlation between mTOR gene expression in PBMCs and cartilage was observed in the end-stage OA patients.
23 OA outpatients in the “Low mTOR expression subset” exhibited significantly lower mTOR gene expression in PBMCs compared to healthy controls.
These “Low mTOR” subset subjects experienced significantly more pain upon walking, and standing and increased total joint stiffness versus “High mTOR” subset, while the latter more often exhibited synovitis.
The protein concentrations of p70-S6K, p21, and caspase 3 in PBMCs were significantly lower in the “Low” subset versus “High” subset and end-stage subjects.
Increases in the expression of mTOR in PBMCs of OA patients are related to disease activity, being associated with synovitis more than with pain.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tchetina, Elena V.& Poole, A. Robin& Zaitseva, Elena M.& Sharapova, Eugeniya P.& Kashevarova, Natalya G.& Taskina, Elena A.…[et al.]. 2013. Differences in Mammalian Target of Rapamycin Gene Expression in the Peripheral Blood and Articular Cartilages of Osteoarthritic Patients and Disease Activity. Arthritis،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-473398
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tchetina, Elena V.…[et al.]. Differences in Mammalian Target of Rapamycin Gene Expression in the Peripheral Blood and Articular Cartilages of Osteoarthritic Patients and Disease Activity. Arthritis No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-473398
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tchetina, Elena V.& Poole, A. Robin& Zaitseva, Elena M.& Sharapova, Eugeniya P.& Kashevarova, Natalya G.& Taskina, Elena A.…[et al.]. Differences in Mammalian Target of Rapamycin Gene Expression in the Peripheral Blood and Articular Cartilages of Osteoarthritic Patients and Disease Activity. Arthritis. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-473398
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473398
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر