Dietary Phenethyl Isothiocyanate Alters Gene Expression in Human Breast Cancer Cells
المؤلفون المشاركون
Morris, Marilyn E.
Moon, Young Jin
Brazeau, Daniel A.
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-09-28
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Phenethyl isothiocyanate (PEITC), a component in cruciferous vegetables, can block chemical carcinogenesis in animal models.
Our objective was to determine the effect of treatment with PEITC on gene expression changes in MCF-7 human breast cancer cells in order to evaluate potential mechanisms involved in its chemopreventive effects.
MCF-7 cells were treated for 48 hours with either PEITC (3 μM) or the vehicle.
Total RNA was extracted from cell membrane preparations, and labeled cDNA's representing the mRNA pool were reverse-transcribed directly from total RNA isolated for use in the microarray hybridizations.
Two specific human GE Array Kits (Superarray Inc.) that both contain 23 marker genes, related to signal transduction pathways or cancer/tumor suppression, plus 2 housekeeping genes (β-actin and GAPDH), were utilized.
Arrays from treated and control cells (n=4 per group) were evaluated using a Student's t-test.
Gene expression was significantly induced for tumor protein p53 (p53), cyclin-dependent kinase inhibitor 1C (p57 Kip2), breast cancer Type 2 early onset (BRCA2), cAMP responsive element binding protein 2 (ATF-2), interleukin 2 (IL-2), heat shock 27 KD protein (hsp27), and CYP19 (aromatase).
Induction of p57 Kip2, p53, BRCA2, IL-2, and ATF-2 would be expected to decrease cellular proliferation and increase tumor suppression and/or apoptosis.
PEITC treatment produced significant alterations in some genes involved in tumor suppression and cellular proliferation/apoptosis that may be important in explaining the chemopreventive effects of PEITC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Moon, Young Jin& Brazeau, Daniel A.& Morris, Marilyn E.. 2010. Dietary Phenethyl Isothiocyanate Alters Gene Expression in Human Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-473485
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Moon, Young Jin…[et al.]. Dietary Phenethyl Isothiocyanate Alters Gene Expression in Human Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-473485
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Moon, Young Jin& Brazeau, Daniel A.& Morris, Marilyn E.. Dietary Phenethyl Isothiocyanate Alters Gene Expression in Human Breast Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-473485
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473485
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر