Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies : Individual Profiling of Prostate Intracrinology
المؤلفون المشاركون
Pierotti, Silvia
Ciccariello, Mauro
Pofi, Riccardo
Barbagallo, Federica
Leonardo, Costantino
Gianfrilli, Daniele
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
In vitro studies reveal that androgens, oestrogens, and their metabolites play a crucial role in prostate homeostasis.
Most of the studies evaluated intraprostatic hormone metabolism using cell lines or preprocessed specimens.
Using an ex vivo model of intact tissue cultures with preserved architecture, we characterized the enzymatic profile of biopsies from patients with benign prostatic hyperplasia (BPH) or cancer (PC), focusing on 17β-hydroxy-steroid-dehydrogenases (17β-HSDs) and aromatase activities.
Samples from 26 men who underwent prostate needle core biopsies (BPH n = 14; PC n = 12) were incubated with radiolabeled 3H-testosterone or 3H-androstenedione.
Conversion was evaluated by TLC separation and beta-scanning of extracted supernatants.
We identified three major patterns of conversion.
The majority of BPHs revealed no active testosterone/oestradiol conversion as opposed to prostate cancer.
Conversion correlated with histology and PSA, but not circulating hormones.
Highest Gleason scores had a higher androstenedion-to-testosterone conversion and expression of 17β-HSD-isoenzymes-3/5.
Conclusions.
We developed an easy tool to profile individual intraprostatic enzymatic activity by characterizing conversion pathways in an intact tissue environment.
In fresh biopsies we found that 17β-HSD-isoenzymes and aromatase activities correlate with biological behaviour allowing for morphofunctional phenotyping of pathology specimens and clinical monitoring of novel enzyme-targeting drugs.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gianfrilli, Daniele& Pierotti, Silvia& Pofi, Riccardo& Leonardo, Costantino& Ciccariello, Mauro& Barbagallo, Federica. 2014. Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies : Individual Profiling of Prostate Intracrinology. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-473687
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gianfrilli, Daniele…[et al.]. Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies : Individual Profiling of Prostate Intracrinology. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-473687
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gianfrilli, Daniele& Pierotti, Silvia& Pofi, Riccardo& Leonardo, Costantino& Ciccariello, Mauro& Barbagallo, Federica. Sex Steroid Metabolism in Benign and Malignant Intact Prostate Biopsies : Individual Profiling of Prostate Intracrinology. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-473687
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473687
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر