Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis
المؤلفون المشاركون
Yu, Peng-Fei
Du, Guangying
Bai, Jing
Kong, Liang
Tian, Jingwei
Zhang, Jianqiao
Fu, Feng-Hua
Wang, Hongbo
Jiang, Yongtao
Pei, Shiqian
Zhang, Lixia
Zhang, Fangxi
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-06-13
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The use of doxorubicin (Dox) was severely constrained by dose-dependent side effects, which might be attenuated by combining a “sensitizer” to decrease its cumulative dosage.
In this study, it was investigated whether ocotillol could enhance the antiproliferation activity of Dox.
MTT assays and xenograft tumor model were firstly conducted to evaluate the effect of ocotillol on the antitumor activity of Dox.
Flow cytometry and Hoechst staining assays were then performed to assess cell apoptosis.
Western blot and real-time PCR were finally used to detect the expression of p53 and its target genes.
Our results showed ocotillol to enhance Dox-induced cell death in p53 wild-type cancer cells.
Compared with Dox alone, Dox with ocotillol (Dox-O) could induce much more cell apoptosis and activate p53 to a much greater degree, which in turn markedly increased expression of proapoptosis genes.
The enhanced cytotoxic activity was partially blocked by pifithrin-α, which might be through attenuating the increased apoptosis.
Furthermore, ocotillol significantly increased the antitumor activity of Dox in A549 xenograft tumor in nude mice.
These findings indicated that ocotillol could potentiate the cytotoxic effect of Dox through p53-dependent apoptosis and suggested that coadministration of ocotillol with Dox might be a potential therapeutic strategy.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Hongbo& Yu, Peng-Fei& Bai, Jing& Zhang, Jianqiao& Kong, Liang& Zhang, Fangxi…[et al.]. 2013. Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473900
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Hongbo…[et al.]. Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-473900
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Hongbo& Yu, Peng-Fei& Bai, Jing& Zhang, Jianqiao& Kong, Liang& Zhang, Fangxi…[et al.]. Ocotillol Enhanced the Antitumor Activity of Doxorubicin via p53-Dependent Apoptosis. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473900
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473900
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر