Uses of Phage Display in Agriculture : Sequence Analysis and Comparative Modeling of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality
المؤلفون المشاركون
Payne, Christina M.
Downie, A. Bruce
Kushwaha, Rekha
المصدر
Computational and Mathematical Methods in Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-07-09
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
A group of intrinsically disordered, hydrophilic proteins—Late Embryogenesis Abundant (LEA) proteins—has been linked to survival in plants and animals in periods of stress, putatively through safeguarding enzymatic function and prevention of aggregation in times of dehydration/heat.
Yet despite decades of effort, the molecular-level mechanisms defining this protective function remain unknown.
A recent effort to understand LEA functionality began with the unique application of phage display, wherein phage display and biopanning over recombinant Seed Maturation Protein homologs from Arabidopsis thaliana and Glycine max were used to retrieve client proteins at two different temperatures, with one intended to represent heat stress.
From this previous study, we identified 21 client proteins for which clones were recovered, sometimes repeatedly.
Here, we use sequence analysis and homology modeling of the client proteins to ascertain common sequence and structural properties that may contribute to binding affinity with the protective LEA protein.
Our methods uncover what appears to be a predilection for protein-nucleic acid interactions among LEA client proteins, which is suggestive of subcellular residence.
The results from this initial computational study will guide future efforts to uncover the protein protective mechanisms during heat stress, potentially leading to phage-display-directed evolution of synthetic LEA molecules.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kushwaha, Rekha& Downie, A. Bruce& Payne, Christina M.. 2013. Uses of Phage Display in Agriculture : Sequence Analysis and Comparative Modeling of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality. Computational and Mathematical Methods in Medicine،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-474053
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kushwaha, Rekha…[et al.]. Uses of Phage Display in Agriculture : Sequence Analysis and Comparative Modeling of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality. Computational and Mathematical Methods in Medicine No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-474053
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kushwaha, Rekha& Downie, A. Bruce& Payne, Christina M.. Uses of Phage Display in Agriculture : Sequence Analysis and Comparative Modeling of Late Embryogenesis Abundant Client Proteins Suggest Protein-Nucleic Acid Binding Functionality. Computational and Mathematical Methods in Medicine. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-474053
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-474053
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر