Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways
المؤلفون المشاركون
Montecucco, Fabrizio
Bertolotto, Maria
Dallegri, Franco
Mach, François
Quercioli, Alessandra
Ottonello, Luciano
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-08-10
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The modulation of CD40L activity might represent a promising therapeutic target to reduce monocyte inflammatory functions in chronic diseases, such as rheumatoid arthritis.
In the present study, we investigated the possible influence of nonsteroidal anti-inflammatory drugs (NSAIDs) on CD40L-induced monocyte survival.
Monocytes were isolated from buffy coats by using Ficoll-Percoll gradients.
Monocyte apoptosis was evaluated by fluorescence microscopy on cytopreps stained with acridine orange or using flow cytometry analysis of Annexin-V and Propidium Iodide staining.
Akt and NF-κB activation was assessed using western blot.
Caspase 3 activity was determined spectrophotometrically.
Among different NSAIDs, only oxaprozin dose-dependently increased apoptosis of CD40L-treated monocytes.
Oxaprozin pro-apoptotic activity was associated with the inhibition of CD40L-triggered Akt and NF-κB phosphorylation and the activation of caspase 3.
In conclusion, our data suggest that oxaprozin-induced apoptosis in CD40L-treated human monocytes is associated with previously unknown cyclooxygenase (COX)-independent pathways.
These intracellular proteins might be promising pharmacological targets to increase apoptosis in CD40L-treated monocytes.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Montecucco, Fabrizio& Bertolotto, Maria& Ottonello, Luciano& Quercioli, Alessandra& Mach, François& Dallegri, Franco. 2009. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-474754
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Montecucco, Fabrizio…[et al.]. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-9.
https://search.emarefa.net/detail/BIM-474754
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Montecucco, Fabrizio& Bertolotto, Maria& Ottonello, Luciano& Quercioli, Alessandra& Mach, François& Dallegri, Franco. Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-9.
https://search.emarefa.net/detail/BIM-474754
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-474754
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر