On-Chip DNA Methylation Analysis Using Osmium Complexation

الملخص EN

The development of a reaction for detecting the presence/absence of one methyl group in a long DNA strand is a chemically and biologically challenging research subject.

A newly designed chemical assay on a chip for the typing of DNA methylation has been developed.

A methylation-detection probe fixed at the bottom of microwells was crosslinked with methylated DNA mediated by osmium complexation and contributes to selective amplification of methylated DNA.