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Phosphorylation and Methylation of Proteasomal Proteins of the Haloarcheon Haloferax volcanii
المؤلفون المشاركون
Zhou, Guangyin
Humbard, Matthew A.
Zuobi-Hasona, Kheir
Maupin-Furlow, Julie A.
Reuter, Christopher J.
المصدر
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-07-08
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Proteasomes are composed of 20S core particles (CPs) of α- and β-type subunits that associate with regulatory particle AAA ATPases such as the proteasome-activating nucleotidase (PAN) complexes of archaea.
In this study, the roles and additional sites of post-translational modification of proteasomes were investigated using the archaeon Haloferax volcanii as a model.
Indicative of phosphorylation, phosphatase-sensitive isoforms of α1 and α2 were detected by 2-DE immunoblot.
To map these and other potential sites of post-translational modification, proteasomes were purified and analyzed by tandem mass spectrometry (MS/MS).
Using this approach, several phosphosites were mapped including α1 Thr147, α2 Thr13/Ser14 and PAN-A Ser340.
Multiple methylation sites were also mapped to α1, thus, revealing a new type of proteasomal modification.
Probing the biological role of α1 and PAN-A phosphorylation by site-directed mutagenesis revealed dominant negative phenotypes for cell viability and/or pigmentation for α1 variants including Thr147Ala, Thr158Ala and Ser58Ala.
An H.
volcanii Rio1p Ser/Thr kinase homolog was purified and shown to catalyze autophosphorylation and phosphotransfer to α1.
The α1 variants in Thr and Ser residues that displayed dominant negative phenotypes were significantly reduced in their ability to accept phosphoryl groups from Rio1p, thus, providing an important link between cell physiology and proteasomal phosphorylation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Humbard, Matthew A.& Reuter, Christopher J.& Zuobi-Hasona, Kheir& Zhou, Guangyin& Maupin-Furlow, Julie A.. 2010. Phosphorylation and Methylation of Proteasomal Proteins of the Haloarcheon Haloferax volcanii. Archaea،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-475032
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Humbard, Matthew A.…[et al.]. Phosphorylation and Methylation of Proteasomal Proteins of the Haloarcheon Haloferax volcanii. Archaea No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-475032
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Humbard, Matthew A.& Reuter, Christopher J.& Zuobi-Hasona, Kheir& Zhou, Guangyin& Maupin-Furlow, Julie A.. Phosphorylation and Methylation of Proteasomal Proteins of the Haloarcheon Haloferax volcanii. Archaea. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-475032
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475032
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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