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D-Glucosamine Promotes Transfection Efficiency during Electroporation
المؤلفون المشاركون
Yamada, Shizuka
Ohara, Naoko
Yanagiguchi, Kajiro
Sugimoto, Kouji
Hayashi, Yoshihiko
Ikeda, Takeshi
Igawa, Kazunari
Kawakubo, Atsushi
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-4، 4ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-11
دولة النشر
مصر
عدد الصفحات
4
التخصصات الرئيسية
الملخص EN
D-Glucosamine is a useful medicament in various fields of medicine and dentistry.
With respect to stability of the cell membrane, it has been reported that bradykinin-induced nociceptive responses are significantly suppressed by the direct application of D-glucosamine.
Electroporation is usually used to effectively introduce foreign genes into tissue culture cells.
Buffers for electroporation with or without D-glucosamine are used in experiments of transfection vectors.
This is the first study to indirectly observe the stability and protection of the osteoblast membrane against both electric stress and gene uptake (the proton sponge hypothesis: osmotic rupture during endosomes prior to fusion with lysosomes) in electroporation with D-glucosamine application.
The transfection efficiency was evaluated as the fluorescence intensity of the transfected green fluorescent protein (GFP) in the cultured cells (osteoblasts; NOS-1 cells).
The transfection efficiency increased over 30% in the electroporation samples treated with D-glucosamine-supplemented buffer after one day.
The membrane absorption of D-glucosamine is the primary mechanism of membrane stress induced by electric stress.
This new function of D-glucosamine is useful and meaningful for developing more effective transformation procedures.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Igawa, Kazunari& Ohara, Naoko& Kawakubo, Atsushi& Sugimoto, Kouji& Yanagiguchi, Kajiro& Ikeda, Takeshi…[et al.]. 2014. D-Glucosamine Promotes Transfection Efficiency during Electroporation. BioMed Research International،Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-475422
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Igawa, Kazunari…[et al.]. D-Glucosamine Promotes Transfection Efficiency during Electroporation. BioMed Research International No. 2014 (2014), pp.1-4.
https://search.emarefa.net/detail/BIM-475422
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Igawa, Kazunari& Ohara, Naoko& Kawakubo, Atsushi& Sugimoto, Kouji& Yanagiguchi, Kajiro& Ikeda, Takeshi…[et al.]. D-Glucosamine Promotes Transfection Efficiency during Electroporation. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-4.
https://search.emarefa.net/detail/BIM-475422
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475422
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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