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Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines)
المؤلفون المشاركون
MacDonald, Margaret H.
Overall, Christopher C.
Klink, Vincent P.
Matthews, Benjamin F.
Alkharouf, Nadim W.
المصدر
Journal of Biomedicine and Biotechnology
العدد
المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-30، 30ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2010-05-19
دولة النشر
مصر
عدد الصفحات
30
التخصصات الرئيسية
الملخص EN
Background.
A comparative microarray investigation was done using detection call methodology (DCM) and differential expression analyses.
The goal was to identify genes found in specific cell populations that were eliminated by differential expression analysis due to the nature of differential expression methods.
Laser capture microdissection (LCM) was used to isolate nearly homogeneous populations of plant root cells.
Results.
The analyses identified the presence of 13,291 transcripts between the 4 different sample types.
The transcripts filtered down into a total of 6,267 that were detected as being present in one or more sample types.
A comparative analysis of DCM and differential expression methods showed a group of genes that were not differentially expressed, but were expressed at detectable amounts within specific cell types.
Conclusion.
The DCM has identified patterns of gene expression not shown by differential expression analyses.
DCM has identified genes that are possibly cell-type specific and/or involved in important aspects of plant nematode interactions during the resistance response, revealing the uniqueness of a particular cell population at a particular point during its differentiation process.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Klink, Vincent P.& Overall, Christopher C.& Alkharouf, Nadim W.& MacDonald, Margaret H.& Matthews, Benjamin F.. 2010. Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-30.
https://search.emarefa.net/detail/BIM-475836
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Klink, Vincent P.…[et al.]. Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-30.
https://search.emarefa.net/detail/BIM-475836
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Klink, Vincent P.& Overall, Christopher C.& Alkharouf, Nadim W.& MacDonald, Margaret H.& Matthews, Benjamin F.. Microarray Detection Call Methodology as a Means to Identify and Compare Transcripts Expressed within Syncytial Cells from Soybean (Glycine max) Roots Undergoing Resistant and Susceptible Reactions to the Soybean Cyst Nematode (Heterodera glycines). Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-30.
https://search.emarefa.net/detail/BIM-475836
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475836
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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