Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms
المؤلفون المشاركون
Lou, Becky
Vigneswaran, Nadarajah
Dubinsky, William P.
Wu, Jean
Engler, David
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-06-07
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
We performed comparative global proteomics analyses of patient-matched primary (686Tu) and metastatic (686Ln) OSCC cells.
The metastatic OSCC 686Ln cells showed greater in vitro migratory/invasive potential and distinct cell shape from their parental primary 686Tu cells.
Ettan DIGE analysis revealed 1316 proteins spots in both cell lines with >85% to be quantitatively similar (<2 folds) between the two cell lines.
However, two protein spots among four serial spots were highly dominant in 686Ln cells.
Mass spectrometry sequencing demonstrated all four spots to be α-tubulin isotypes.
Further analysis showed no significant quantitative difference in the α-tubulin between the two cell lines either at mRNA or protein levels.
Thus, two distinct isoforms of α-tubulin, probably due to posttranslational modification, were associated with metastatic 686Ln cells.
Immunofluorescence demonstrated remarkable differences in the cytosolic α-tubulin distribution patterns between the two cells.
In 686Tu cells, α-tubulin proteins formed a normal network composed of filaments.
In contrast, α-tubulin in 686Ln cells exhibited only partial cytoskeletal distribution with the majority of the protein diffusely distributed within the cytosol.
Since α-tubulin is critical for cell shape and mobility, our finding suggests a role of α-tubulin isoforms in acquisition of metastatic phenotype and represents potential target for therapeutic intervention.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lou, Becky& Engler, David& Dubinsky, William P.& Wu, Jean& Vigneswaran, Nadarajah. 2012. Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms. Journal of Oncology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-475879
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lou, Becky…[et al.]. Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms. Journal of Oncology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-475879
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lou, Becky& Engler, David& Dubinsky, William P.& Wu, Jean& Vigneswaran, Nadarajah. Acquiring Metastatic Competence by Oral Squamous Cell Carcinoma Cells Is Associated with Differential Expression of α-Tubulin Isoforms. Journal of Oncology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-475879
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475879
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر