Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth
المؤلفون المشاركون
Haastert-Talini, Kirsten
Krings, Laura
Bozkurt, Ahmet
Brook, Gary A.
van Neerven, Sabien G. A.
Vogt, Michael
Pallua, Norbert
Tolba, René H.
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-05-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
A variety of new bioartificial nerve guides have been tested preclinically for their safety and nerve regeneration supporting properties.
So far, only a limited number of biomaterials have been tested in humans since the step from preclinical work to a clinical application is challenging.
We here present an in vitro model with human Schwann cells (hSCs) as an intermediate step towards clinical application of the nerve guide Perimaix, a collagen-based microstructured 3D scaffold containing numerous longitudinal guidance channels for directed axonal growth.
hSCs were seeded onto different prototypes of Perimaix and cultivated for 14 days.
hSC adhered to the scaffold, proliferated, and demonstrated healthy Schwann cell morphology (spindle shaped cell bodies, bipolar oriented processes) not only at the surface of the material, but also in the deeper layers of the scaffold.
The general well-being of the cells was quantitatively confirmed by low levels of lactate dehydrogenase release into the culture medium.
Moreover, conditioned medium of hSCs that were cultivated on Perimaix was able to modify neurite outgrowth from sensory dorsal root ganglion neurons.
Overall these data indicate that Perimaix is able to provide a matrix that can promote the attachment and supports process extension, migration, and proliferation of hSC.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
van Neerven, Sabien G. A.& Krings, Laura& Haastert-Talini, Kirsten& Vogt, Michael& Tolba, René H.& Brook, Gary A.…[et al.]. 2014. Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth. BioMed Research International،Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-476097
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
van Neerven, Sabien G. A.…[et al.]. Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth. BioMed Research International No. 2014 (2014), pp.1-13.
https://search.emarefa.net/detail/BIM-476097
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
van Neerven, Sabien G. A.& Krings, Laura& Haastert-Talini, Kirsten& Vogt, Michael& Tolba, René H.& Brook, Gary A.…[et al.]. Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-13.
https://search.emarefa.net/detail/BIM-476097
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-476097
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر