Comprehensive Transcriptome Study to Develop Molecular Resources of the Copepod Calanus sinicus for Their Potential Ecological Applications

المؤلفون المشاركون

Li, Hongjun
Sun, Fanyue
Yang, Zhi
Yang, Qing

المصدر

BioMed Research International

العدد

المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-12، 12ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2014-05-20

دولة النشر

مصر

عدد الصفحات

12

التخصصات الرئيسية

الطب البشري

الملخص EN

Calanus sinicus Brodsky (Copepoda, Crustacea) is a dominant zooplanktonic species widely distributed in the margin seas of the Northwest Pacific Ocean.

In this study, we utilized an RNA-Seq-based approach to develop molecular resources for C.

sinicus.

Adult samples were sequenced using the Illumina HiSeq 2000 platform.

The sequencing data generated 69,751 contigs from 58.9 million filtered reads.

The assembled contigs had an average length of 928.8 bp.

Gene annotation allowed the identification of 43,417 unigene hits against the NCBI database.

Gene ontology (GO) and KEGG pathway mapping analysis revealed various functional genes related to diverse biological functions and processes.

Transcripts potentially involved in stress response and lipid metabolism were identified among these genes.

Furthermore, 4,871 microsatellites and 110,137 single nucleotide polymorphisms (SNPs) were identified in the C.

sinicus transcriptome sequences.

SNP validation by the melting temperature (Tm)-shift method suggested that 16 primer pairs amplified target products and showed biallelic polymorphism among 30 individuals.

The present work demonstrates the power of Illumina-based RNA-Seq for the rapid development of molecular resources in nonmodel species.

The validated SNP set from our study is currently being utilized in an ongoing ecological analysis to support a future study of C.

sinicus population genetics.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Yang, Qing& Sun, Fanyue& Yang, Zhi& Li, Hongjun. 2014. Comprehensive Transcriptome Study to Develop Molecular Resources of the Copepod Calanus sinicus for Their Potential Ecological Applications. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-476098

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Yang, Qing…[et al.]. Comprehensive Transcriptome Study to Develop Molecular Resources of the Copepod Calanus sinicus for Their Potential Ecological Applications. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-476098

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Yang, Qing& Sun, Fanyue& Yang, Zhi& Li, Hongjun. Comprehensive Transcriptome Study to Develop Molecular Resources of the Copepod Calanus sinicus for Their Potential Ecological Applications. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-476098

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-476098