Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance
المؤلفون المشاركون
Abdallah, Cosette
Sergeant, Kjell
Dumas-Gaudot, Eliane
Renaut, Jenny
المصدر
International Journal of Plant Genomics
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-11-20
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Two-dimensional gel electrophoresis (2-DE) is widely applied and remains the method of choice in proteomics; however, pervasive 2-DE-related concerns undermine its prospects as a dominant separation technique in proteome research.
Consequently, the state-of-the-art shotgun techniques are slowly taking over and utilising the rapid expansion and advancement of mass spectrometry (MS) to provide a new toolbox of gel-free quantitative techniques.
When coupled to MS, the shotgun proteomic pipeline can fuel new routes in sensitive and high-throughput profiling of proteins, leading to a high accuracy in quantification.
Although label-based approaches, either chemical or metabolic, gained popularity in quantitative proteomics because of the multiplexing capacity, these approaches are not without drawbacks.
The burgeoning label-free methods are tag independent and suitable for all kinds of samples.
The challenges in quantitative proteomics are more prominent in plants due to difficulties in protein extraction, some protein abundance in green tissue, and the absence of well-annotated and completed genome sequences.
The goal of this perspective assay is to present the balance between the strengths and weaknesses of the available gel-based and -free methods and their application to plants.
The latest trends in peptide fractionation amenable to MS analysis are as well discussed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Abdallah, Cosette& Dumas-Gaudot, Eliane& Renaut, Jenny& Sergeant, Kjell. 2012. Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance. International Journal of Plant Genomics،Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-476150
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Abdallah, Cosette…[et al.]. Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance. International Journal of Plant Genomics No. 2012 (2012), pp.1-17.
https://search.emarefa.net/detail/BIM-476150
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Abdallah, Cosette& Dumas-Gaudot, Eliane& Renaut, Jenny& Sergeant, Kjell. Gel-Based and Gel-Free Quantitative Proteomics Approaches at a Glance. International Journal of Plant Genomics. 2012. Vol. 2012, no. 2012, pp.1-17.
https://search.emarefa.net/detail/BIM-476150
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-476150
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر