Inhibition of Rat Muscle and Liver Phosphofructokinases by High Doses of Ethanol

المؤلفون المشاركون

Barkovsky, Eugene Victorovich
Lelevich, Sergey Vladimirovich
Khrustalev, Vladislav Victorovich

المصدر

Biochemistry Research International

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-11-13

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الكيمياء

الملخص EN

Activities of both rat muscle and liver phosphofructokinases are significantly inhibited after a single ethanol intake in the dose of 2.5 g per kg of body weight.

This inhibitory effect is indirect, since ethanol in concentration (50 mM) close to that established after 2.5 g per kg of body weight intake cannot decrease their activities in vitro.

Inhibition of liver phosphofructokinase activity after the 5.0 g per kg ethanol intake may be direct, since liver phosphofructokinase activity decreases in vitro when ethanol is added to supernatants of rat liver tissue in 100 mM concentration.

According to the results of molecular docking, ethanol at high concentrations can be bound by adenine-binding pocket of the allosteric ADP-binding site of liver phosphofructokinase (Asp543, Phe308, Phe538, and Phe671) and its activation by ADP can be blocked by C2H5OH molecule.

Direct inhibition of muscle phosphofructokinase activity, probably due to the binding of ethanol to the similar ADP-binding site, is possible when the concentration of ethanol (500 mM) is much higher than the level which can be established in living cells.

So, inhibition of muscle phosphofructokinase activity after a single 5.0 g per kg intake is indirect and probably linked with the inhibition of the enzyme by elevated citrate and phosphoenolpyruvate levels.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Lelevich, Sergey Vladimirovich& Khrustalev, Vladislav Victorovich& Barkovsky, Eugene Victorovich. 2013. Inhibition of Rat Muscle and Liver Phosphofructokinases by High Doses of Ethanol. Biochemistry Research International،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476196

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Lelevich, Sergey Vladimirovich…[et al.]. Inhibition of Rat Muscle and Liver Phosphofructokinases by High Doses of Ethanol. Biochemistry Research International No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-476196

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Lelevich, Sergey Vladimirovich& Khrustalev, Vladislav Victorovich& Barkovsky, Eugene Victorovich. Inhibition of Rat Muscle and Liver Phosphofructokinases by High Doses of Ethanol. Biochemistry Research International. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-476196

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-476196