DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation
المؤلفون المشاركون
Marcondes, A. Mario
Borinstein, Scott C.
Black, Jennifer
Grady, William M.
Chen, Xi
Patel, Nikul
Lawlor, Elizabeth R.
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-09-12
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
The role of aberrant DNA methylation in Ewing sarcoma is not completely understood.
The methylation status of 503 genes in 52 formalin-fixed paraffin-embedded EWS tumors and 3 EWS cell lines was compared to human mesenchymal stem cell primary cultures (hMSCs) using bead chip methylation analysis.
Relative expression of methylated genes was assessed in 5-Aza-2-deoxycytidine-(5-AZA)-treated EWS cell lines and in a cohort of primary EWS samples and hMSCs by gene expression and quantitative RT-PCR.
129 genes demonstrated statistically significant hypermethylation in EWS tumors compared to hMSCs.
Thirty-six genes were profoundly methylated in EWS and unmethylated in hMSCs.
5-AZA treatment of EWS cell lines resulted in upregulation of expression of hundreds of genes including 162 that were increased by at least 2-fold.
The expression of 19 of 36 candidate hypermethylated genes was increased following 5-AZA.
Analysis of gene expression from an independent cohort of tumors confirmed decreased expression of six of nineteen hypermethylated genes (AXL, COL1A1, CYP1B1, LYN, SERPINE1,) and VCAN.
Comparing gene expression and DNA methylation analyses proved to be an effective way to identify genes epigenetically regulated in EWS.
Further investigation is ongoing to elucidate the role of these epigenetic alterations in EWS pathogenesis.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Patel, Nikul& Black, Jennifer& Chen, Xi& Marcondes, A. Mario& Grady, William M.& Lawlor, Elizabeth R.…[et al.]. 2012. DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation. Sarcoma،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476506
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Patel, Nikul…[et al.]. DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation. Sarcoma No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-476506
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Patel, Nikul& Black, Jennifer& Chen, Xi& Marcondes, A. Mario& Grady, William M.& Lawlor, Elizabeth R.…[et al.]. DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation. Sarcoma. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-476506
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-476506
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر