Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes : Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating
المؤلفون المشاركون
Iida, Fumie
Kojima, Ryo
Miura, Toshihide
Ise, Keiko
Sogawa, Kazuyuki
Katayama, Katsuhiro
Satoh, Mamoru
Kiyokawa, Iwao
Nomura, Fumio
المصدر
International Journal of Proteomics
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-09-12
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء
الملخص EN
One possible factor determining recovery of trace amount of protein biomarker candidates during proteome analyses could be adsorption on urine tubes.
This issue, however, has not been well addressed so far.
Recently, a new technical device of surface coating by poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-co-n-butyl methacrylate (BMA)) (poly(MPC-co-BMA)) has been developed mainly to prevent the adsorption of plasma proteins.
We assessed whether conventionally used urine tubes adsorb trace amount of urinary proteins and, if any, whether the surface coating by poly(MPC-co-BMA) can minimize the adsorption.
Proteinuric urine samples were kept in poly(MPC-co-BMA)-coated and noncoated urine tubes for 15 min and possibly adsorbed proteins and/or peptides onto urine tubes were analyzed by SDS-PAGE, 2-DE, and the MALDI-TOF MS.
It was found that a number of proteins and/or peptides adsorb on the conventionally used urine tubes and that surface coating by poly(MPC-co-BMA) can minimize the adsorption without any significant effects on routine urinalysis test results.
Although it remains to be clarified to what extent the protein adsorption can modify the results of urinary proteome analyses, one has to consider this possible adsorption of urinary proteins when searching for trace amounts of protein biomarkers in urine.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kiyokawa, Iwao& Sogawa, Kazuyuki& Ise, Keiko& Iida, Fumie& Satoh, Mamoru& Miura, Toshihide…[et al.]. 2011. Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes : Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating. International Journal of Proteomics،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-476736
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kiyokawa, Iwao…[et al.]. Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes : Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating. International Journal of Proteomics No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-476736
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kiyokawa, Iwao& Sogawa, Kazuyuki& Ise, Keiko& Iida, Fumie& Satoh, Mamoru& Miura, Toshihide…[et al.]. Adsorption of Urinary Proteins on the Conventionally Used Urine Collection Tubes : Possible Effects on Urinary Proteome Analysis and Prevention of the Adsorption by Polymer Coating. International Journal of Proteomics. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-476736
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-476736
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر