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O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin
المؤلفون المشاركون
Zenteno, Edgar
Lascurain, Ricardo
Estrada-Parra, Sergio
Chávez, Raúl
Estrada-Garcia, Iris
Martínez-Cairo, Salvador
Méndez-Reguera, Aniela
Jiménez-Martínez, María C.
Gorocica, Patricia
المصدر
Clinical and Developmental Immunology
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-09-24
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
O-glycosidically-linked glycans have been involved in development, maturation, homing, and immune regulation in T cells.
Previous reports indicate that Amaranthus leucocarpus lectin (ALL), specific for glycans containing galactose-N-acetylgalactosamine and N-acetylgalactosamine, recognizes human naïve CD27+CD25+CD4+ T cells.
Our aim was to evaluate the phenotype of CD4+ T cells recognized by ALL in peripheral blood mononuclear cells obtained from healthy volunteers.
CD4+ T cells were isolated by negative selection using magnetic beads-labeled monoclonal antibodies; the expression of T regulatory cell phenotypic markers was assessed on ALL-recognized cells.
In addition, IL-4, IL-10, IFN-γ, and TGF-β intracellular production in ALL+ cells was also evaluated.
The analyses of phenotypic markers and intracellular cytokines were performed through flow cytometry.
ALL-recognized CD4+ T cells were mainly CD45RA+, CCR7+ cells.
Although 52±10% CD25+Foxp3+ cells were positive to ALL, only 34±4% of ALL+ cells corresponded to CD25+Foxp3− cells.
Intracellular cytokines in freshly obtained ALL+CD4+ T cells exhibited 8% of IL-4, 15% of IL-10, 2% of IFN-γ, and 15% of TGF-β, whereas ALL−CD4+ T cells depicted 1% of IL-4, 2% of IL-10, <1% of IFN-γ, and 6% of TGF-β.
Our results show that galactose-N-acetylgalactosamine and N-galactosamine-bearing CD4+ T cells expressed phenotypic markers of NnTreg cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Jiménez-Martínez, María C.& Lascurain, Ricardo& Méndez-Reguera, Aniela& Estrada-Parra, Sergio& Estrada-Garcia, Iris& Gorocica, Patricia…[et al.]. 2013. O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-477084
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Jiménez-Martínez, María C.…[et al.]. O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin. Clinical and Developmental Immunology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-477084
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Jiménez-Martínez, María C.& Lascurain, Ricardo& Méndez-Reguera, Aniela& Estrada-Parra, Sergio& Estrada-Garcia, Iris& Gorocica, Patricia…[et al.]. O-Glycosylation of NnTreg Lymphocytes Recognized by the Amaranthus leucocarpus Lectin. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-477084
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-477084
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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