An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines

المؤلفون المشاركون

Donati, Chiara
Rigogliuso, Salvatrice
Vittorelli, Maria Letizia
Salamone, Monica
Cassarà, Donata
Bruni, Paola
Taverna, Simona

المصدر

Journal of Oncology

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-05-24

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

الأمراض
الطب البشري

الملخص EN

Expression of sphingosine kinase-1 (SphK-1) correlates with a poor survival rate of tumor patients.

This effect is probably due to the ability of SphK-1 to be released into the extracellular medium where it catalyzes the biosynthesis of sphingosine-1-phosphate (S1P), a signaling molecule endowed with profound proangiogenic effects.

SphK-1 is a leaderless protein which is secreted by an unconventional mechanism.

In this paper, we will show that in human hepatocarcinoma Sk-Hep1 cells, extracellular signaling is followed by targeting the enzyme to the cell surface and parallels targeting of FGF-2 to the budding vesicles.

We will also show that SphK-1 is present in a catalitycally active form in vesicles shed by SK-Hep1 and human breast carcinoma 8701-BC cells.

The enzyme substrate sphingosine is present in shed vesicles where it is produced by neutral ceramidase.

Shed vesicles are therefore a site for S1P production in the extracellular medium and conceivably also within host cell following vesicle endocytosis.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Rigogliuso, Salvatrice& Donati, Chiara& Cassarà, Donata& Taverna, Simona& Salamone, Monica& Bruni, Paola…[et al.]. 2010. An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines. Journal of Oncology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-477307

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Rigogliuso, Salvatrice…[et al.]. An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines. Journal of Oncology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-477307

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Rigogliuso, Salvatrice& Donati, Chiara& Cassarà, Donata& Taverna, Simona& Salamone, Monica& Bruni, Paola…[et al.]. An Active Form of Sphingosine Kinase-1 Is Released in the Extracellular Medium as Component of Membrane Vesicles Shed by Two Human Tumor Cell Lines. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-477307

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-477307