Psychrophilic Enzymes : From Folding to Function and Biotechnology

المؤلف

Feller, Georges

المصدر

Scientifica

العدد

المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-28، 28ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2013-01-17

دولة النشر

مصر

عدد الصفحات

28

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأمراض

الملخص EN

Psychrophiles thriving permanently at near-zero temperatures synthesize cold-active enzymes to sustain their cell cycle.

Genome sequences, proteomic, and transcriptomic studies suggest various adaptive features to maintain adequate translation and proper protein folding under cold conditions.

Most psychrophilic enzymes optimize a high activity at low temperature at the expense of substrate affinity, therefore reducing the free energy barrier of the transition state.

Furthermore, a weak temperature dependence of activity ensures moderate reduction of the catalytic activity in the cold.

In these naturally evolved enzymes, the optimization to low temperature activity is reached via destabilization of the structures bearing the active site or by destabilization of the whole molecule.

This involves a reduction in the number and strength of all types of weak interactions or the disappearance of stability factors, resulting in improved dynamics of active site residues in the cold.

These enzymes are already used in many biotechnological applications requiring high activity at mild temperatures or fast heat-inactivation rate.

Several open questions in the field are also highlighted.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Feller, Georges. 2013. Psychrophilic Enzymes : From Folding to Function and Biotechnology. Scientifica،Vol. 2013, no. 2013, pp.1-28.
https://search.emarefa.net/detail/BIM-477546

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Feller, Georges. Psychrophilic Enzymes : From Folding to Function and Biotechnology. Scientifica No. 2013 (2013), pp.1-28.
https://search.emarefa.net/detail/BIM-477546

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Feller, Georges. Psychrophilic Enzymes : From Folding to Function and Biotechnology. Scientifica. 2013. Vol. 2013, no. 2013, pp.1-28.
https://search.emarefa.net/detail/BIM-477546

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-477546